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AIDS Res Hum Retroviruses. 1999 Feb 10;15(3):285-91.

Reverse transcriptase activity and particle production in B lymphoblastoid cell lines established from lymphocytes of patients with multiple sclerosis.

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1
Department of Medical Microbiology, University of Aarhus, Denmark. mikrtc@svfcd.aau.dk

Abstract

We have established spontaneously formed B lymphoblastoid cell lines from long-term cultured peripheral blood mononuclear cells (PBMNCs) from multiple sclerosis (MS) patients. The MS cell lines actively produce retrovirus-like particles as well as Epstein-Barr virus (EBV). Using three different variations of the highly sensitive polymerase chain reaction (PCR)-based assays for the detection of reverse transcriptase (RT) activity, we have verified the retroviral origin of the retrovirus-like particles that are produced in very low amounts by the MS cell lines.

PMID:
10052759
DOI:
10.1089/088922299311466
[Indexed for MEDLINE]
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