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J Chromatogr B Biomed Sci Appl. 1999 Jan 22;721(2):321-6.

High-performance liquid chromatographic assay with a simple extraction procedure for sensitive quantification of mycophenolic acid in rat and human plasma.

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  • 1Transplantation Immunology, Department of Cardiothoracic Surgery, Stanford University Medical School, CA 94305-5247, USA.


We describe a novel sensitive and simplified gradient HPLC assay for quantification of the immunosuppressant mycophenolic acid (MPA) in rat and human plasma. In contrast to previously reported MPA assays, our method used a single step extraction comprising addition of acetonitrile, which contained phenolphthalein glucoronic acid as internal standard, for protein precipitation. Linearity: 0.1-100 microg/ml (r2>0.999), mean recoveries: MPA 98.0%, internal standard 105.2%, mean intra-day precision: 4.3%, mean day-to-day precision: 4.3%, mean day-to-day accuracy: -1.5%. Sensitivity was sufficient to allow for quantification of mycophenolic acid in as little as 50 microl plasma.

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