Neuroprotective effect of PMA against H₂O₂-induced toxicity on rat primary neurons. Rat primary neurons were exposed to oxidative stress injury induced by H₂O₂ (75 μM), and neuron survival was estimated 24 h after the beginning of the experiment (A and B). Concentration-dependent biological effects of PMA were observed against H₂O₂ toxicity when PMA was added 24 h before exposure of H₂O₂ on hippocampal (A) and cortical (B) neurons. In C, hippocampal neurons were pretreated with PMA (0.1 and 1.0 μM) before the addition of different concentrations of H₂O₂ (30, 100, and 300 μM). Western blot analysis showed the expression of PKC in hippocampal neuronal cultures after PMA treatment (D). (E) An inactive enantiomer of PMA, 4α-PMA, was added in the same conditions as for PMA. Hippocampal neurons were treated by using identical conditions as described above and 5.0 μM of the HO inhibitor (SnPPIX) was added at the same time as PMA. (F) The effect of a PKC inhibitor, bisindolylmaleimide, on the neuroprotective effect of PMA in hippocampal neuronal cultures.

PMA neuroprotection is lost in hippocampal cultures of HO2^−/− mice. Primary hippocampal neurons from wild-type (A) and HO2 knockout (B) mice were cultured similarly as rat neuronal cultures. Neurons were treated with various concentrations of H₂O₂ (60, 80, and 100 μM). PMA (0.1 and 1.0 μM) was added 24 h before the addition of H₂O₂. Neuronal survival was assessed 24 h later.

In vitro phosphorylation of hHO2. (A) Time-dependent effect on phosphorylation of GST–hHO2 incubated with [³²P]ATP and purified brain PKC. The upper band shows the phosphorylation of PKC itself and the lower band depicts the phosphorylation of GST–hHO2. (B) HO2 phosphorylation in HEK 293 cells stably overexpressing hHO2. The top row shows incorporation of [³²P]ATP into HO2 after phosphorylation induced by treatment with PMA (0 and 0.1 μM). HO2 was immunoprecipitated with two different antibodies (Left, SPA297; Right, our antibody), then subjected to electrophoresis and transferred to nitrocellulose membrane. Quantification of the [³²P]HO2 with the PhosphorImager reveals an increased of 1.63 (Left) and 1.72 (Right) times after PMA treatment over the control. The Lower row was obtained after Western blot analysis on the same nitrocellulose membrane with an anti-phosphoserine antibody, suggesting that immunoprecipitated HO2 was phosphorylated on serine residues. Right indicates that HO2 was loaded in similar amounts.

Photomicrograph of rat primary hippocampal neurons incubated with an antibody against BR showing BR accumulation after PMA treatment. (A) Control staining performed without the addition of the primary antibody. (B–H) Staining with the anti-BR under different stimuli. Cells were treated with 0.1 μM PMA for various time periods (0, 1, 6, or 24 h) (B–E), with 1.0 μM PMA for 24 h (F), with 0.1 μM inactive enantiomer 4α-PMA (G), or with 0.1 μM in combination with 5.0 μM HO inhibitor SnPPIX (H).

Neuroprotective effect of bilirubin on H₂O₂-induced toxicity. (A) Increasing concentrations of free BR were added to rat primary hippocampal neurons before addition of 75 μM H₂O₂. (B) Increasing concentrations of BR complexed with human serum albumin (BR–HSA was added to neurons). An equivalent concentration HSA of albumin was added. Neuron survival was estimated 24 h after the beginning of the experiment.