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Oncogene. 1999 Feb 18;18(7):1495-501.

Physical and functional interactions between the transcription factor PU.1 and the coactivator CBP.

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1
Department of Cell Genetics, Sasaki Institute, Tokyo, Japan.

Abstract

Yeast two-hybrid system was employed to isolate novel proteins that physically interact with PU.1, a member of Ets family transcription factors. Sequence analyses of several isolated clones positive for beta-galactosidase activity revealed that one of these clones was confirmed to encode a transcriptional coactivator, CREB binding protein (CBP). GST binding assay showed that the interacting sites were located at the transcriptional activation domain of PU.1 through 74-122 and the region spanning residues 1283-1915 of CBP. CBP potentiated PU.1-mediated transcription of the reporter gene driven by the multimerized PU.1-binding sites, suggesting that CBP functions as a coactivator for PU.1. Considering that CBP is a limited cellular component to function as a coactivator for several transcription factors, CBP may mediate synergistic and antagonistic interactions between PU.1 and other transcription factors during the process of hematopoietic cell differentiation.

PMID:
10050886
DOI:
10.1038/sj.onc.1202427
[Indexed for MEDLINE]
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