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Biochem Biophys Res Commun. 1999 Feb 16;255(2):340-6.

Intracellular localization of SNAP-23 to endosomal compartments.

Author information

1
Department of Biochemistry, University of Kentucky College of Medicine, Chandler Medical Center, Lexington 40536, USA.

Abstract

We have reexamined the intracellular localization of the ubiquitously expressed target membrane SNAP receptor (t-SNARE), SNAP-23. While SNAP-23 appears on the plasma membrane, in the cell types examined there is a significant pool associated with endosomal compartments. Immuno-staining and expression of green fluorescent protein-tagged SNAP-23, show that it has a punctate, perinuclear localization in HepG2 and HT4 cells. This distribution overlaps significantly with transferrin receptor and slightly with the late endosome/lysosomal protein LAMP-1. The localization of SNAP-23 changes as HepG2 cells polarize. Initially it is concentrated at sites of cell-cell contact and then almost exclusively to the apical (or bile canalicular) domain of the cell. These data are consistent with a role for SNAP-23 in both endosome-plasma membrane trafficking as well as endosome-endosome transport.

PMID:
10049710
DOI:
10.1006/bbrc.1999.0173
[Indexed for MEDLINE]

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