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Nucleic Acids Res. 1999 Mar 15;27(6):1555-7.

Rapid modification of bacterial artificial chromosomes by ET-recombination.

Author information

1
Gene Expression Program, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.

Abstract

We present a method to modify bacterial artificial chromosomes (BACs) resident in their host strain. The method is based on homologous recombination by ET-cloning. We have successfully modified BACs at two distinct loci by recombination with a PCR product containing homology arms of 50 nt. The procedure we describe here is rapid, was found to work with high efficiency and should be applicable to any BAC modification desired.

PMID:
10037821
PMCID:
PMC148353
DOI:
10.1093/nar/27.6.1555
[Indexed for MEDLINE]
Free PMC Article

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