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EMBO J. 1999 Feb 15;18(4):916-25.

The role of RBF in the introduction of G1 regulation during Drosophila embryogenesis.

Author information

1
MGH Cancer Center, Building 149, 13th Street, Charlestown, MA 02129, USA. wdu@fben-may.basd.uchicago.edu

Abstract

The first appearance of G1 during Drosophila embryogenesis, at cell cycle 17, is accompanied by the down-regulation of E2F-dependent transcription. Mutant alleles of rbf were generated and analyzed to determine the role of RBF in this process. Embryos lacking both maternal and zygotic RBF products show constitutive expression of PCNA and RNR2, two E2F-regulated genes, indicating that RBF is required for their transcriptional repression. Despite the ubiquitous expression of E2F target genes, most epidermal cells enter G1 normally. Rather than pausing in G1 until the appropriate time for cell cycle progression, many of these cells enter an ectopic S-phase. These results indicate that the repression of E2F target genes by RBF is necessary for the maintenance but not the initiation of a G1 phase. The phenotype of RBF-deficient embryos suggests that rbf has a function that is complementary to the roles of dacapo and fizzy-related in the introduction of G1 during Drosophila embryogenesis.

PMID:
10022834
PMCID:
PMC1171184
DOI:
10.1093/emboj/18.4.916
[Indexed for MEDLINE]
Free PMC Article

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