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Life Sci. 2019 Sep 1;232:116670. doi: 10.1016/j.lfs.2019.116670. Epub 2019 Jul 19.

Evaluation of protective effects of non-selective cannabinoid receptor agonist WIN 55,212-2 against the nitroglycerine-induced acute and chronic animal models of migraine: A mechanistic study.

Author information

1
Department of Neurology, Kashan University of Medical Sciences, Kashan, Iran. Electronic address: mv.elahe@gmail.com.
2
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: baradaranv941@mums.ac.ir.
3
Department of Neurology, Kashan University of Medical Sciences, Kashan, Iran.
4
Department of Physiology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
5
Physiology Research Centre, Kashan University of Medical Sciences, Kashan, Iran; Department of Pharmacology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran. Electronic address: banafshe57@hotmail.com.
6
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: askariv941@mums.ac.ir.

Abstract

AIM:

Migraine is a neurological debilitating disorder. Previous studies have shown that cannabinoid receptor agonists have analgesic effects in various models of pain. In this study, therefore, we investigated anti-nociceptive effects of WIN 55,212-2, and the role of either CB1 or CB2 receptors in nitroglycerine (NTG)-induced animal model of migraine.

METHODS:

The present study was conducted on both male and female rats receiving NTG (10 mg/kg, i.p.) to induce acute (single dose of NTG) and chronic (repetitive doses of NTG) models of migraine. Additionally, three groups received WIN 55,212-2 (0.33, 1, 3 mg/kg, i.p.) 45 min before behavioral tests. Additionally, AM251 and AM630 (CB1 and CB2 receptor antagonist, respectively, 1 mg/kg, i.p.) were used to evaluate the possible involvement of CB1 and CB2 receptors during the protective effects of WIN 55,212-2.

KEY FINDINGS:

We found that NTG (10 mg/kg, i.p.) in both acute and chronic models increased sensitivity to pain. In acute model, we found that WIN 55,212-2 (almost high doses) decreases the level of pain mainly through CB1 receptor due to CB1 antagonist abrogates its protective effects, however, in formalin test CB2 receptors also had crucial roles in both phases at 3 mg/kg of WIN 55,212-2. In chronic model, WIN 55,212-2 (0.33, 1 and 3 mg/kg) significantly attenuated NTG-induced hyperalgesia through both CB1 and CB2 receptors.

SIGNIFICANCE:

Our data supported the argument that activation of CB1 and CB2 receptors by WIN 55,212-2 may be considered a new medication for migraine, however in lack of each receptor leads to different responses from deletion to the reduction of analgesic effects.

KEYWORDS:

Cannabinoid; Hyperalgesia; Migraine; Nitroglycerin; WIN 55,212-2

PMID:
31330139
DOI:
10.1016/j.lfs.2019.116670

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