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Nanotechnol Sci Appl. 2017 Jun 29;10:115-121. doi: 10.2147/NSA.S133415. eCollection 2017.

Antibacterial effect of silver nanoparticles in Pseudomonas aeruginosa.

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Biotechnology Interunits Pos Graduation Program, University of Sao Paulo - USP, Butantan Institute, Institute for Technological Research - IPT, Sao Paulo, SP, Brazil.
Industrial Biotechnology Laboratory, Bionanomanufacture Nucleus, Institute for Technological Research - IPT, São Paulo, SP, Brazil.


Pseudomonas aeruginosa has great intrinsic antimicrobial resistance limiting the number of effective antibiotics. Thus, other antimicrobial agents such as silver nanoparticles (AgNPs) are considered potential agents to help manage and prevent infections. AgNPs can be used in several applications against bacteria resistant to common antibiotics or even multi-resistant bacteria such as P. aeruginosa. This study assessed the antimicrobial activity of commercial 10 nm AgNPs on two hospital strains of P. aeruginosa resistant to a large number of antibiotics and a reference strain from a culture collection. All strains were susceptible to 5 µg/mL nanoparticles solution. Reference strains INCQS 0230 and P.a.1 were sensitive to AgNPs at concentrations of 1.25 and 0.156 µg/mL, respectively; however, this was not observed for hospital strain P.a.2, which was more resistant to all antibiotics and AgNPs tested. Cytotoxicity evaluation indicated that AgNPs, up to a concentration of 2.5 µg/mL, are very safe for all cell lines tested. At 5.0 µg/mL, AgNPs had a discrete cytotoxic effect on tumor cells HeLa and HepG2. Results showed the potential of using AgNPs as an alternative to conventional antimicrobial agents that are currently used, and a perspective for application of nanosilver with antibiotics to enhance antimicrobial activity.


AgNPs; Pseudomonas aeruginosa; antimicrobial activity; bacterial resistance; silver nanoparticles

Conflict of interest statement

Disclosure The authors report no conflicts of interest in this work.

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