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PLoS One. 2014 Dec 2;9(12):e113591. doi: 10.1371/journal.pone.0113591. eCollection 2014.

The relative biological effectiveness for carbon and oxygen ion beams using the raster-scanning technique in hepatocellular carcinoma cell lines.

Author information

1
Department of Radiation Oncology, Im Neuenheimer Feld 400, 69120, Heidelberg, Germany; Department of Radiation Oncology, Klinikum rechts der Isar, Technische Universität München, Isma-ninger Str. 22, 81675, Munich, Germany.
2
Department of Radiation Oncology, Im Neuenheimer Feld 400, 69120, Heidelberg, Germany.
3
Heidelberg Ion Beam Therapy Center (HIT), Im Neuenheimer Feld 450, 69120, Heidelberg, Germany.
4
Department of Radiation Oncology, Klinikum rechts der Isar, Technische Universität München, Isma-ninger Str. 22, 81675, Munich, Germany.

Abstract

BACKGROUND:

Aim of this study was to evaluate the relative biological effectiveness (RBE) of carbon (12C) and oxygen ion (16O)-irradiation applied in the raster-scanning technique at the Heidelberg Ion beam Therapy center (HIT) based on clonogenic survival in hepatocellular carcinoma cell lines compared to photon irradiation.

METHODS:

Four human HCC lines Hep3B, PLC, HepG2 and HUH7 were irradiated with photons, 12C and 16O using a customized experimental setting at HIT for in-vitro trials. Cells were irradiated with increasing physical photon single doses of 0, 2, 4 and 6 Gy and heavy ion-single doses of 0, 0.125, 0.5, 1, 2, 3 Gy (12C and 16O). SOBP-penetration depth and extension was 35 mm +/-4 mm and 36 mm +/-5 mm for carbon ions and oxygen ions respectively. Mean energy level and mean linear energy transfer (LET) were 130 MeV/u and 112 keV/um for 12C, and 154 MeV/u and 146 keV/um for 16O. Clonogenic survival was computated and relative biological effectiveness (RBE) values were defined.

RESULTS:

For all cell lines and both particle modalities α- and β-values were determined. As expected, α-values were significantly higher for 12C and 16O than for photons, reflecting a steeper decline of the initial slope of the survival curves for high-LET beams. RBE-values were in the range of 2.1-3.3 and 1.9-3.1 for 12C and 16O, respectively.

CONCLUSION:

Both irradiation with 12C and 16O using the raster-scanning technique leads to an enhanced RBE in HCC cell lines. No relevant differences between achieved RBE-values for 12C and 16O were found. Results of this work will further influence biological-adapted treatment planning for HCC patients that will undergo particle therapy with 12C or 16O.

PMID:
25460352
PMCID:
PMC4252049
DOI:
10.1371/journal.pone.0113591
[Indexed for MEDLINE]
Free PMC Article

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