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Photomed Laser Surg. 2008 Jun;26(3):197-202. doi: 10.1089/pho.2007.2125.

Low-intensity laser irradiation improves the mitochondrial dysfunction of C2C12 induced by electrical stimulation.

Author information

1
Laboratory of Laser Sports Medicine, College of Sports Sciences, South China Normal University, Guangzhou, China. xuxy@scnu.edu.cn

Abstract

OBJECTIVE:

We investigated the effects of electrical stimulation and low-intensity laser (LIL) energy on the mitochondrial function of cultured C2C12 myotubes in order to find a dosage that could be used to improve the function of mitochondria, and then rehabilitate exercise-induced damage and fatigue.

BACKGROUND DATA:

Many other studies in the past demonstrated that LIL had a cytoprotective effect, and a recent study also found that LIL could reduce muscular fatigue during tetanic contractions in rats.

METHODS:

Cultured C2C12 myotubes were subjected to electrical stimulation or/and LIL irradiation at various intensities. Reactive oxygen species (ROS) were detected with a fluorescent probe (DCFH-DA) and mitochondrial function was assessed with an MTT assay.

RESULTS:

The results showed that electrical stimulation at 20 ms, 5 Hz, and 45 V for 75 min can induce mitochondrial dysfunction in cultured C2C12 myotubes. Electrical stimulation-induced mitochondrial dysfunction was improved, but degeneration occurred with LIL at doses of 0.33-8.22 and 11.22-14.16 J/cm2, respectively, and these changes were markedly increased with LIL at 0.33 and 1.34 J/cm2, respectively.

CONCLUSIONS:

We conclude that treatment of myotubes with the proper dosage of LIL irradiation significantly diminished production of ROS and restored mitochondrial function, and this may provide a foundation for the use of photobiomodulation to treat exercise-induced mitochondrial dysfunction or skeletal muscular fatigue.

PMID:
18484910
DOI:
10.1089/pho.2007.2125
[Indexed for MEDLINE]

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