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Genetics. 2005 Jul;170(3):1423-6. Epub 2005 May 23.

A new reversion assay for measuring all possible base pair substitutions in Saccharomyces cerevisiae.

Author information

1
Department of Biology, Emory University, Atlanta, Georgia 30322, USA.

Abstract

A TRP5-based reversion system that allows the rates of all possible base pair substitutions to be measured when the TRP5 locus is in both orientations relative to a defined origin of replication has been developed. This system should be useful for a wide variety of mutation and repair studies in yeast.

PMID:
15911571
PMCID:
PMC1451166
DOI:
10.1534/genetics.105.042697
[Indexed for MEDLINE]
Free PMC Article

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