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J Renin Angiotensin Aldosterone Syst. 2016 Jul-Sep;17(3):1470320316663327. doi: 10.1177/1470320316663327.

miR-205 mediates the inhibition of cervical cancer cell proliferation using olmesartan.

Author information

1
1 Tianjin Central Hospital of Gynecology Obstetrics, Tianjin 300052, China.
2
2 Departments of Gynecology and Obstetrics,Tianjin Medical University General Hospital, Tianjin, China.

Abstract

OBJECTIVE:

The renin-angiotensin-aldosterone system has become known as a prerequisite for tumor angiogenesis that is now recognized as a crucial step in the development of tumors, including cervical cancer. The Ang II-AT1R pathway is known to play an important role in tumor angiogenesis. MicroRNAs (miRNAs) are a class of small, regulating RNAs that participate in tumor genesis, differentiation and proliferation. The current study focused on the anti-tumor mechanism of olmesartan, a novel angiotensin II antagonist, on cervical cancer cells.

MATERIALS AND METHODS:

qRT-PCR and Western blot were used to demonstrate the effect of olmesartan on miR-205 and VEGF-A expression. miR-205 mimics and VEGF-A shRNA plasmid were separately transfected into HeLa and Siha cells to further validate the function of miR-205 and VEGF-A in cervical cancer cell proliferation.

RESULTS:

It was found that olmesartan could upregulate miR-205 and inhibit VEGF-A expression in HeLa and Siha cells. In addition, VEGF-A was proven to be a target gene of miR-205.

CONCLUSION:

This result provides a new idea on the anti-tumor mechanism of olmesartan, which may be used as a novel therapeutic target of cervical cancer.

KEYWORDS:

Olmesartan; VEGF-A; cervical cancer; miR-205; proliferation

PMID:
28304186
PMCID:
PMC5843885
DOI:
10.1177/1470320316663327
[Indexed for MEDLINE]
Free PMC Article

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