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J Chemother. 2016 Aug;28(4):314-20. doi: 10.1179/1973947815Y.0000000055.

Angiotensin II receptor antagonist olmesartan and NF-kappaB inhibitor as cytotoxic and apoptotic agents in MCF-7 human cell line.

Author information

1
a Department of Pharmacology, School of Medicine , Mashhad University of Medical Sciences , Iran.
2
b Pharmacological Research Center of Medicinal Plants, School of Medicine , Mashhad University of Medical Sciences , Iran.
3
c Medical Toxicology Research Center, School of Medicine , Mashhad University of Medical Sciences , Iran.

Abstract

OBJECTIVE:

Over expression of renin-angiotensin system (RAS) and nuclear factor-kappaB (NF-kappaB) have major role in many cancers. In this study, role of angiotensin II (Ag II) and NF-kappaB pathway in breast cancer cell line (MCF-7) proliferation were studied using olmesartan (as a novel Ag II antagonist) and Bay11-7082 (as NF-kappaB inhibitor).

MATERIALS AND METHODS:

Cells were treated with different concentrations of olmesartan and Bay11-7082.Cell proliferation was determined by 4, 5-Dimethylthiazol-2-yl, 2, 5-diphenyl tetrazolium (MTT) assay. Apoptotic cells were evaluated using PI staining of DNA fragmentation.

RESULTS:

Olmesartan and Bay11-7082 decreased cell viability. Combination of olmesartan with Bay11-7082 also decreased cell viability as compared with single agent treatments. Results showed that apoptosis is involved in olmesartan and Bay11-7082-induced toxicity.

CONCLUSION:

Olmesartan and Bay11-7082 inhibit the MCF-7 cells growth indicating RAS and NF-kappaB pathway blockade lead to cytotoxicity and apoptosis induction against tumour cells. So ARBs and NF-kappaB pathway inhibitors could be considered as anticancer drugs in future.

KEYWORDS:

Bay11-7082; Cytotoxicity; MCF-7; Olmesartan

PMID:
26138656
DOI:
10.1179/1973947815Y.0000000055
[Indexed for MEDLINE]

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