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| Status |
Public on Nov 28, 2013 |
| Title |
Differential expression of miRNAs in Brassica napus root following infection with Plasmodiophora brassicae |
| Platform organisms |
Physcomitrium patens; Picea glauca; Picea sitchensis; Pinus taeda; Liriodendron tulipifera; Persea americana; Populus tremuloides; Populus trichocarpa; Populus trichocarpa x Populus deltoides; Malus domestica; Prunus persica; Lupinus luteus; Medicago truncatula; Phaseolus vulgaris; Phaseolus coccineus; Manihot esculenta; Ricinus communis; Nicotiana sylvestris; Nicotiana tabacum; Nicotiana benthamiana; Petunia x hybrida; Ipomoea batatas; Lactuca sativa; Hordeum vulgare; Oryza rufipogon; Oryza sativa; Oryza brachyantha; Oryza nivara; Cenchrus americanus; Sorghum bicolor; Lolium arundinaceum; Phleum pratense; Lotus japonicus; Cenchrus ciliaris; Ipomoea nil; Prunus armeniaca; Peridictyon sanctum; Panicum virgatum; Populus tremula x Populus tremuloides; Rorippa indica; Oryza barthii; Nasturtium officinale; Nuphar advena; Prunus salicina; Hordeum vulgare subsp. vulgare; Populus tremula; Oenocarpus bataua; Malcolmia maritima |
| Sample organism |
Brassica napus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Canola (oilseed rape, Brassica napus L.), is susceptible to infection by the biotrophic protist Plasmodiophora brassicae, the causal agent of clubroot. To understand the roles of microRNAs (miRNAs) during the post-transcriptional regulation of disease initiation and progression, we have characterized the changes in miRNA expression profiles in canola roots during clubroot disease development and have compared these to uninfected roots. Two different stages of clubroot development were targeted in this miRNA profiling study: an early time of 10-dpi for disease initiation and a later 20-dpi, by which time the pathogen had colonized the roots (as evident by visible gall formation and histological observations). P. brassicae responsive miRNAs were identified and validated by qRT-PCR of miRNAs and the subsequent validation of the target mRNAs through starBase degradome analysis, and through 5’ RLM-RACE. This study identifies putative miRNA-regulated genes with roles during clubroot disease initiation and development. Putative target genes identified in this study included: transcription factors (TFs), hormone-related genes, as well as genes associated with plant stress response regulation such as cytokinin, auxin/ethylene response elements. The results of our study may assist in elucidating the role of miRNAs in post-transcriptional regulation of target genes during disease development and may contribute to the development of strategies to engineer durable resistance to this important phytopathogen.
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| Overall design |
In this miRNA-microarray experiment a total of 4 samples were analyzed with their 3 biological replicates. In which 2 samples C 10 DAY and C 20 DAY was used as referrence contols.
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| Contributor(s) |
Verma SS, Kav N |
| Citation(s) |
24497962 |
| Submission date |
Oct 23, 2013 |
| Last update date |
Feb 10, 2014 |
| Contact name |
Sanjay Gupta |
| E-mail(s) |
gxs44@case.edu
|
| Phone |
2163686162
|
| Organization name |
Case Western Reserve University
|
| Department |
Department of Urology
|
| Lab |
Cancer Research
|
| Street address |
10900 Euclid Avenue
|
| City |
Cleveland |
| State/province |
Ohio |
| ZIP/Postal code |
44106 |
| Country |
USA |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA223665 |
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