 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 01, 2020 |
| Title |
Defining Murine Monocyte Differentiation into Colonic and Ileal Macrophages |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Monocytes are circulating short-lived macrophage precursors that are recruited on demand from the blood to sites of inflammation and challenge. In steady state, classical monocytes give rise to vasculature-resident cells that patrol the luminal side of the endothelium. In addition, classical monocytes feed macrophage compartments of selected organs, including barrier tissues, such as the skin and intestine, as well as the heart. Monocyte differentiation under conditions of inflammation has been studied in considerable detail. In contrast, monocyte differentiation under non-inflammatory conditions remains less well understood. Here we took advantage of a combination of cell ablation and precursor engraftment to investigate the generation of gut macrophages from monocytes. Collectively, we identify factors associated with the gradual adaptation of monocytes to tissue residency. Moreover, comparison of monocyte differentiation into the colon and ileum-resident macrophages revealed the graduated acquisition of gut segment-specific gene expression signatures.
|
| |
|
| Overall design |
Monocyte-derived macrophages were sorted from ileum and colon. Sorted cells were subjected to RNAseq in triplicates. In total, 3 time points were investigated per organ. Further, naïve monocytes and tissue-resident macrophages were sorted for RNAseq (triplicates).
|
| |
|
| Contributor(s) |
Gross-Vered M, Trzebanski S, Shemer A, Bernshtein B, Curato C, Stelzer G, Salame T, David E, Boura-Halfon S, Chappell-Maor L, Leshkovitz D, Jung S |
| Citation(s) |
31916932 |
| Submission date |
Nov 25, 2019 |
| Last update date |
Jan 21, 2020 |
| Contact name |
Steffen Jung |
| Organization name |
Weizmann Institute of Science
|
| Department |
Department of Immunology
|
| Street address |
234 Herzl Street
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (32)
|
|
| Relations |
| BioProject |
PRJNA591465 |
| SRA |
SRP233166 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE140919_20150629B_MG_exons_raw.txt.gz |
1.7 Mb |
(ftp)(http) |
TXT |
| GSE140919_20161218_BB_exons_raw.txt.gz |
2.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...