Silencing genes with RNAi
RNA interference (RNAi) is mechanism for sequence-specific gene silencing that promises to
revolutionize experimental biology and may have important therapeutic applications. See the reference
listed below for some review articles on RNAi.
Applications of RNAi
Functional genomics: large-scale screening of mutant phenotypes
Therapeutic intervention: targeting pathogens, cancer and inherited genetic disorders
Agriculture: making desease resistant plants and plants with improved nutritional and handling characteristics
RNAi resources at the NCBI
The NCBI is developing databases to store the sequences of RNAi reagents and experimental results
generated using those reagents. This page provides access to some sample data.
The Probe database is a comprehensive collection of oligonucleotide probes used in a variety of
applications, including RNAi, gene expression assays, variation analysis, and molecular diagnosis. Each
probe entry may be linked to experimental results and published articles.
Probe is included as a
database within the Entrez system.
How to submit data
Data submission tools are still under development. In the meantime, if you have data to submit, you
may contact the database staff directly.
|Latest annual review
Dykxhoorn DM, Lieberman J. THE SILENT REVOLUTION: RNA Interference as Basic Biology, Research Tool, and Therapeutic.
Annu Rev Med. 2005;56:401-23.
|RNAi in groups of organisms|
Bantounas I, Phylactou LA et al. RNA interference and the use of small interfering RNA to study gene function in mammalian systems. J Mol Endocrinol. 2004 Dec;33(3):545-57.
Susi P, Hohkuri M et al. Characteristics of RNA silencing in plants: similarities and differences across kingdoms.
Plant Mol Biol. 2004 Jan;54(2):157-74.
Cottrell TR, Doering TL. Silence of the strands: RNA interference in eukaryotic pathogens.
Trends Microbiol. 2003 Jan;11(1):37-43.
Tan FL, Yin JQ. RNAi, a new therapeutic strategy against viral infection.
Cell Res. 2004 Dec;14(6):460-6.
Andres AJ. Flying through the genome: a comprehensive study of functional genomics using RNAi in Drosophila.
Trends Endocrinol Metab. 2004 Aug;15(6):243-7.
|Types of RNAi sequences|
Svoboda P. Long dsRNA and silent genes strike back:RNAi in mouse oocytes and early embryos.
Cytogenet Genome Res. 2004;105(2-4):422-34.
|Short dsRNA (siRNA)|
Gilmore IR, Fox SP et al. The design and exogenous delivery of siRNA for post-transcriptional gene silencing.
J Drug Target. 2004 Jul;12(6):315-40.
More about RNAi in PubMed
View gene silencing terms
How it works
The RNAi Consortium (TRC)
CGAP RNAi at NCI
Drosophila RNAi screening center
Arabidopsis Small RNA Project
Other RNAi resources
|siRNA design algorithms evaluation|
Sætrom P, Snøve O Jr. A comparison of siRNA efficacy predictors.
Biochem Biophys Res Commun. 2004 Aug 13;321(1):247-53.
Companies developing RNAi technology|
(companies marked by asterisk have their own siRNA design tools)
- Alnylam Pharma (Ribopharma) - Cambridge, MA, USA
- Ambion - Austin, TX, USA*
- Benitec - Queensland, Australia
- Cenix Bioscience - Dresden, Germany
- Dharmacon Inc. - Lafayette, CO, USA*
- Eurogentec - Liège, Belgium
- International Therapeutics - Seattle, WA, USA
- GeneLink - Hawthorne, NY, USA*
- Intradigm Corp. - Rockville, MD, USA
- Invitrogen (Sequitur) - Carlsbad, CA, USA*
- Mirus Bio Corp. - Madison, WI, USA
- Open Biosystems - Hunstville, AL, USA
- Proligo LLC - Boulder, CO, USA
- Qiagen (Xeragon) - Venlo, Netherland*
- The MWG - Ebersberg, Germany*
- Santaris Pharma - Copenhagen, Denmark
- Sirna Therapeutics - Boulder, CO, USA