Healthy Donor Controls for Immune Evaluation

Bioenergetic dysfunction is an archetypical finding in critical illness, particularly among patients with sepsis. A central feature of sepsis is the shift towards pathologic immunosuppression predisposing to secondary infections and ineffectual organ recovery. However, the extent to which metabolic abnormalities contribute to immune cell dysregulation in critically ill patients with and without sepsis remains unclear. To investigate this, we collected peripheral blood mononuclear cells (PBMCs) from medical and surgical ICU patients at Vanderbilt University Medical Center 48-72 hours after ICU admission, as well as community-matched healthy controls, with all studies conducted under IRB approval. We performed single cell RNA-sequencing using the Chromium Next GEM Single Cell 5' HT Kit v2 (10x Genomics) on a subset of 9 healthy controls, 19 ICU patients without sepsis, and 19 ICU patients with sepsis, generating a comprehensive dataset of 644,147 cells providing a detailed transcriptional map of critical illness. The healthy control sequences are provided here; the ICU patient sequences are provided in dbGaP phs004258.v1.p1.

• Study Weblinks:
  • NCBI GEO
• Study Design:
  • Control Set
• Study Type:
  • Control Set
• Total number of consented subjects: 9
• Subject Sample Telemetry Report (SSTR)

• BioProject
• PubMed
• Sequence Read Archive
• PMC

Inclusion Criteria:

1. Adults (≥18 years of age).

Exclusion Criteria:

1. Pregnant.
2. Presence of an active acute or chronic infection.
3. History of significant anemia.
4. Receiving T cell-directed immunosuppression (e.g., tacrolimus, azathioprine, mycophenolate).
5. Receiving any antineoplastic or immunomodulatory therapy.
6. Active hematologic malignancy.
7. Prior allogeneic stem cell transplant.
8. Current participation in another study where cumulative blood draw volume exceeds 550 mL within an eight-week period.

• Primary Phenotype: Healthy Volunteers
• Leukocytes, Mononuclear

• Principal Investigator
  • Matthew T. Stier, MD, PhD. Vanderbilt University Medical Center, Nashville, TN, USA.
• Co-Investigator
  • Jeffrey C. Rathmell, PhD. Vanderbilt University Medical Center, Nashville, TN, USA.