Transcriptome and TCR Sequencing of T Cells from Metastectomies

T cells were sorted from metastatic tumors (colon, rectal, breast, anal, melanoma primary histologies) following tumor dissociation and single-cell sequencing was performed (RNAseq and TCRseq). For samples from tumors that had been previously studied, cells were analyzed comparing known neoantigen-reactive TCR-expressing cells to other T cells. For samples without previously identified neoantigen reactivity, TCRs were predicted on the basis of T cell transcriptomic states. To determine candidate neoantigen screening, exome and RNA sequencing were performed on fragments of matched tumors from which T cells were sequenced

• Study Design:
  • Case Set
• Study Type:
  • Case Set
  • RNA Sequencing
  • Single Cell Analysis
• Total number of consented subjects: 15
• Subject Sample Telemetry Report (SSTR)

• BioProject
• PubMed
• Sequence Read Archive
• MeSH
• Gene
• PMC
• Clinical Trials
  • NCT00001823
  • NCT00068003

Informed consent was obtained and documented in accordance with the Declaration of Helsinki. Patients were required to be 18 years of age or older, to have measurable metastatic disease, to be of good ECOG performance status, and to be free of systemic infections.

• Primary Phenotype: Colonic Neoplasms
• Rectal Neoplasms
• Breast Neoplasms
• Anus Neoplasms
• Melanoma

• CXCL13

• Principal Investigator
  • Steven A. Rosenberg, MD, PhD. National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
• Co-Investigator
  • Frank J. Lowery, PhD. National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.