INSIGHT (Intervention Nurses Start Infants Growing on Healthy Trajectories) Cohort

This is a study of the biological risk factors for children enrolled in the INSIGHT (Intervention Nurses Start Infants Growing on Healthy Trajectories) study. INSIGHT is a randomized, controlled trial testing a responsive parenting intervention designed for the primary prevention of childhood obesity against a safety control. These first-born children were deeply studied in this time period with data collected on a wide variety of variables including mode of delivery, gender, weight and height (collected at 7 time points), medication usage, diet information, and maternal health information (gestational weight gain, gestational diabetes, smoking during pregnancy).

This study includes a survey of the oral and gut microbiota from 226 mother-child dyads. It was designed to investigate the relationship between a cross-sectional view of the child's microbiome (at two years of age) and the patterns of growth between birth and 2 years. Microbiome samples from children (buccal swab and stool sample) and their mothers (buccal swab) are collected at the children's 2-year clinical research visit. Stool metabolites have also been assayed for a subset of the 226 children using 1H-NMR.

Additionally, we genotyped the same individuals for SNPs across the genome.

• Study Weblinks:
  • NCBI BioProject PRJNA420339
• Study Design:
  • Prospective Longitudinal Cohort
• Study Type:
  • Cohort
• dbGaP estimated ancestry using GRAF-pop
• Total number of consented subjects: 457
• Subject Sample Telemetry Report (SSTR)

Inclusion/exclusion criteria for participants in this study are explained in detail in Paul et al. BMC Pediatrics. 2014 (PMID:25037579).

Briefly, individuals were first-born children delivered at Penn State Milton S. Hershey Medical Center in Hershey, Pennsylvania. These individuals were full-term (>37 weeks gestation) singletons. Mothers were English-speaking, primiparous, >20 years of age, and residing within 50 miles of the medical center. Exclusion criteria include a plan for the infant to be adopted or moved from Central Pennsylvania within 3 years, a prenatal ultrasound demonstrating intrauterine growth retardation, a newborn weight of <2500 grams, or if the mother or infant had significant health issues affecting study participation.

Microbiome molecular data for this study is available on the Sequence Read Archive (SRA) under BioProject number PRJNA420339.

• Primary Phenotype: Pediatric Obesity

• BioProject
• PubMed
• PMC
• Clinical Trials
  • NCT01167270

• Principal Investigator
  • Kateryna D. Makova, PhD. Penn State University, University Park, PA, USA.
• Funding Sources
  • R01DK088244. National Institutes of Health, Bethesda, MD, USA.
  • R01DK99364. National Institutes of Health, Bethesda, MD, USA.
  • UL1TR000127. National Institutes of Health, Bethesda, MD, USA.
  • Tobacco Settlement and CURE funds. Pennsylvania Department of Health, Harrisburg, PA, USA.