Jump to: Authorized Access | Attribution | Authorized Requests

Study Description

We identified germline heterozygous mutations in CTLA4 in members of four families with severe immune dysregulation. Human CTLA4 haploinsufficiency caused dysregulation of FoxP3+ regulatory T (Treg) cells and lymphocytic infiltration of target organs, mimicking Ctla4 homozygous mice. Patients also exhibited a B cell phenotype, with progressive loss of B cells and accumulation of autoreactive CD21lo B cells. This study demonstrates a critical quantitative role for CTLA-4 in human immune homeostasis.

Authorized Access
Publicly Available Data
  Link to other NCBI resources related to this study
Study Inclusion/Exclusion Criteria

Inclusion criteria: Combination of hypogammaglobulinemia and/or CD4 lymphopenia, and infiltration of at least one non-lymphoid organ [brain, lungs and gastrointestinal tract] with lymphocytic aggregates, autoimmune cytopenia.

Exclusion criteria: Any known previously identified genetic defects for immune dysregulation and lymphoproliferation

Molecular Data
TypeSourcePlatformNumber of Oligos/SNPsSNP Batch IdComment
Whole Exome Capture Agilent SureSelect 38Mb N/A N/A
Whole Exome Capture Agilent SureSelect Human All Exon v3 - 50Mb N/A N/A
Next-Generation Sequencing Illumina HiSeq 2500 N/A N/A
Next-Generation Sequencing Thermo Fisher Scientific SOLiD Next-Generation Sequencing N/A N/A
Selected Publications
Diseases/Traits Related to Study (MeSH terms)
Authorized Data Access Requests
Study Attribution
  • Principal Investigator
    • Gulbu Uzel, MD. National Institutes of Health, Bethesda, MD, USA.
  • Co-Principal Investigators
    • Michael J. Lenardo, MD. National Institutes of Health, Bethesda, MD, USA.
    • Thomas A. Fleisher, MD. National Institutes of Health, Bethesda, MD, USA.
    • Stuart G. Tangye, PhD. Garvan Institute of Medical Research, Sydney, Australia.
    • Eric Meffre, PhD. Yale University School of Medicine, New Haven, CT, USA.
    • Dat. Q. Tran, MD. University of Texas Medical School, Houston, TX, USA.
    • Steven M. Holland, MD. National Institutes of Health, Bethesda, MD, USA.
  • Funding Sources
    • Intramural Research. National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
    • Intramural Research Program, NIH , CC. National Institutes of Health, Bethesda, MD, USA.
    • 1066694 (to S.G.T.). National Health and Medical Research Council of Australia (Cancer Council NSW), Canberra, Australia.
    • 1027400 (to S.G.T.). National Health and Medical Research Council of Australia (Cancer Council NSW), Canberra, Australia.
    • 1016953 (to S.G.T.). National Health and Medical Research Council of Australia (Cancer Council NSW), Canberra, Australia.
    • RG 12-08 (to S.G.T.). National Health and Medical Research Council of Australia (Cancer Council NSW), Canberra, Australia.
    • AI071087 (to E.M.). National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
    • AI095848 (to E.M.). National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
    • AI061093 (to E.M.). National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
    • 5R01HL113304-01 (to D.Q.T.). National Institutes of Health, Bethesda, MD, USA.