PMC

Schematic representation of the components of the neurovascular unit at the level of brain capillaries and post-capillary venules. Drawings of the individual cell types were adapted from Servier Medical Art (http://smart.servier.com/), licensed under a Creative Common Attribution 3.0 Generic License.

Schematic representation of the meningeal layers. The different layers and cellular composition of the meningeal layers are displayed. Tight junctions are highlighted between the arachnoid barrier cells as parallel lines, with adherens junctions, desmosomes and gap junctions also being represented. The shapes of the cell types were adapted from Servier Medical Art (http://smart.servier.com/), licensed under a Creative Common Attribution 3.0 Generic License.

Schematic representation of the junctional complexes of the BCSFB at the choroid plexus. In similarity to the BBB, the proteins that compose the tight and adherens junctions of the choroid plexus BCSFB are connected to the cytoskeleton via intracellular scaffolding proteins and are localized in the apical part of the choroid plexus epithelial cells. The shapes of the proteins were adapted from Servier Medical Art (http://smart.servier.com/), licensed under a Creative Common Attribution 3.0 Generic License.

Schematic representation of the junctional complexes of the BBB. The proteins that compose the tight and adherens junctions are connected to the cytoskeleton via intracellular scaffolding proteins, ZO-1, ZO-2 and AF-6. Despite being expressed at low levels by the BBB endothelial cells [], the subcellular location and function of claudin-12 remains to be defined. The forms of the individual proteins were adapted from Servier Medical Art (http://smart.servier.com/), licensed under a Creative Common Attribution 3.0 Generic License.

Representative images of the cranial and spinal cord window 2P-IVM imaging of the claudin-5-GFP reporter mouse. (a) The cranial window was placed over the right hemisphere of the mouse brain as depicted on the insert. Second harmonic generation in blue derives from collagen fibers in the dura mater. The strong GFP signal visible throughout the brain endothelial cells allows for imaging of the vessels along the entire vascular tree. (b) Cranial window region from (a) after removal of the dura mater. (c) Cervical spinal cord window. High magnification image showing the dorsal vein and branching veins. Scale bars = 100µm.

Visualization of CNS and meningeal endothelial AJs using the VE-Cadherin-GFP reporter mouse. (a) Cranial window allowing to visualize meningeal, subarachnoid, subpial and cortical vascular VE-cadherin-GFP+ AJs. (b) Cranial window preparation from (a) highlighting blood vessels after i.v. injection of TRITC Dextran. (c) Overlay of a and b and the second harmonic generation of the collagen fibers in the dura allow to distinguish VE-cadherin-GFP+ TRITC+ blood vessels and VE-cadherin-GFP+ TRITC^neg lymphatic vessels in the dura mater. Examples of blood and lymphatic vessels of similar caliber size are highlighted with a yellow and red arrowhead, respectively. The cranial window was placed over the right hemisphere of the mouse brain as depicted in the insert below (a). Top row—3D stack, bottom—XZ maximum intensity projection of 20 µm along Y at the cross section highlighted at the top. Scale bars = 90µm.