PMC

Principal Component Analysis of wild and farmed gilthead seabream populations based on neutral microsatellite allele frequencies. Population groups are indicated in the coloured circles corresponding to their cluster membership identified by Structure (see Fig. ). Population abbreviations are as in Table . Historical wild samples (09HW) were excluded from the analysis.

Hierarchical population structure based on Structure analyses of gilthead seabream genotypes at 19 neutral microsatellite loci. The first hierarchical partitioning divided the dataset into six clusters (K = 6). In the second partitioning step, only clusters that showed additional subdivision are presented. Vertical coloured lines represent individual admixture coefficients. Black horizontal lines divide individuals from different sampling sites. Population abbreviations are as in Table .

Upper: Spatial particle distribution on 15 May 2016 obtained in the Ichthyop – Adriatic ROMS simulation from the sourced tuna-aquaculture surroundings near islands of (a) Brač and (b) Ugljan. Source populations are marked with red dots (16AB – Brač, 16AK – Ugljan). The black squares, representing areas of success, are depicted around natural nursery grounds (green dots) where YoY were sampled (16JR – Raša; 16JP – Pantana). Lower (c): Spatial particle distributions on 15 May 2016 obtained in the Ichthyop – ASHELF2 ROMS simulation from the sourced tuna-aquaculture surrounding near the island of Brač. The source population is marked with a red dot (16AB – Brač). The black square, representing the area of success, is depicted around a natural nursery ground (green dot point) (16JP – Pantana). The figure has been created using MATLAB 2014a (www.mathworks.com) and GIMP 2.8.16 (www.gimp.org) software.

Adriatic Sea bathymetry with sampling locations of wild adults gilthead seabream (WH, Brač channel; WT, Tar Bay; WN, Novigrad Sea; WR, Rovinj; WU, Unije; WV, Velebit Channel; WD, Dubrovnik), wild young-of-the year (YOY) (JP, Pantana River; JN, Neretva River; JR, Raša River; JB, Maslinova Bay), farm-associated adults (AK, Mrdina; AB, Maslinova Bay; AV, Vrgada) and farmed adults (FC, Budava Bay; FF-FFb, Maslinova Bay; FI, Mali Ston Bay; FI-FG, Lamljana Bay; FFa, Lim Bay). Tuna-aquaculture sites (red dots) and nurseries (green dots) were involved in spatial particle distribution simulation. Blue points represent locations of HF radars at Cape Ražanj, island of Brač (R1 43.319688°N, 16.408743°E), and at Cape Stončica, island of Vis (R2; 43.072141°N, 16.254157°E). Contours are drawn for 250, 500 and 1000 m depths. On the map of the Mediterranean Sea (upper right corner), the Adriatic ROMS domain is shown in the red rectangle, and the ASHELF2 ROMS domain in the blue rectangle. More information about population abbreviations and sampling years are provided in Table . The figure has been created using MATLAB 2014a (www.mathworks.com) and GIMP 2.8.16 (www.gimp.org) software.