PMC

Diaporthe hungariae (CBS 143353). a–c. Colonies on MEA, PDA and OA, respectively; d. conidiomata sporulating on PNA; e. conidiogenous cells; f. alpha conidia. — Scale bars = 10 μm.

Diaporthe bohemiae (CBS 143347). a–c. Colonies on MEA, PDA and OA, respectively; d. conidiomata sporulating on PNA; e. conidiogenous cells; f. alpha conidia. — Scale bars = 10 μm.

Diaporthe celeris (CBS 143349). a–c. Colonies on MEA, PDA and OA, respectively; d. conidiomata sporulating on OA; e. conidiophores; f. conidiogenous cells; g. alpha conidia; h. beta conidia. — Scale bars = 10 μm.

Diaporthe hispaniae (CBS 143351). a–c. Colonies on MEA, PDA and OA, respectively; d. conidiomata sporulating on PDA; e. conidiogenous cells; f. alpha conidia; g. beta conidia. — Scale bars = 10 μm.

a–e. Natural and f–g. artificial symptoms on V. vinifera with associated Diaporthe species. a–c. Lesions of Phomopsis cane and leaf spot on shoot: a. initial symptoms (courtesy Alessandro Vitale); b. severe symptoms on green; c. dead shoot (courtesy José Luis Ramos Sáez de Ojer). — d–e. Cane bleaching (courtesy José Luis Ramos Sáez de Ojer). — f–g. External and internal discoloration of shoot inoculated with D. hispaniae (CPC 30323).

Consensus phylogram of 3 862 trees resulting from a Bayesian analysis of the combined ITS, tub2, his3, tef1 and cal sequence alignments of Diaporthe spp. Bootstrap support values and Bayesian posterior probability values are indicated at the nodes. The asterisk symbol (*) represents full support (1/100). Substrate and country of origin are listed next to the strain numbers. Ex-type isolates are indicated in bold. The novel species are shown in red text. The tree was rooted to Diaporthella corylina (CBS 121124).

Consensus phylogram of 86 082 trees resulting from a Bayesian analysis of the combined ITS, tub2, his3, tef1 and cal sequence alignments of the D. eres complex. Bootstrap support values and Bayesian posterior probability values are indicated at the nodes. The asterisk symbol (*) represents full support (1/100). Substrate and country of origin are listed next to the strain numbers. Ex-type isolates are indicated in bold. The novel species are shown in red text. The tree was rooted to Diaporthe toxica (CBS 534.93).