PMC

Downregulated expression of TP73-AS1 inhibits the WNT/β-catenin signaling pathway in gastric cancer cells (A) Images of blots were captured and (B) relative protein expression was evaluated at 48 h when cells were transfected with si-NC or si-TP73-AS1 in MGC-803 cells. (C) Images of blots were captured and (D) relative protein expression was evaluated at 48 h when cells were transfected with si-NC or si-TP73-AS1 in BGC-823 cells. *P<0.05, vs. si-NC. TCF4, transcription factor 4; si, small interfering RNA; NC, negative control.

Downregulation of the expression of TP73-AS1 inhibits cell proliferation ability in gastric cancer. Expression of TP73-AS1 was significantly downregulated following transfection of cells with si-TP73-AS1-1 or si-TP73-AS1-2 in (A) MGC-803 and (B) BGC-823 cells. Cell viability was detected at 0, 24, 48 and 72 h when cells were transfected with si-NC or si-TP73-AS1 in (C) MGC-803 and (D) BGC-823 cells. *P<0.05, vs. si-NC. si, small interfering RNA; NC, negative control.

Downregulation of the expression of TP73-AS1 inhibits cell colony formation and cell invasion in gastric cancer. (A) Images of colonies; (B) numbers of cell colonies were calculated at 48 h when the cells were transfected with si-NC or si-TP73-AS1-in MGC-803 or BGC-823 cells. Magnification, ×200. (C) Images of the cell invasion assay; (D) numbers of invading cells were evaluated at 48 h when cells were transfected with si-NC or si-TP73-AS1 in MGC-803 or BGC-823 cells, Magnification, ×200. *P<0.05, vs. si-NC. si, small interfering RNA; NC, negative control.

Expression of TP73-AS1 is significantly upregulated in GC tissues compared with adjacent normal tissues. (A) TP73-AS1 was significantly upregulated in 64 pairs of GC tissues when compared with adjacent normal tissues using reverse transcription-quantitative polymerase chain reaction analysis. (B) Disease-free survival rate was statistically higher in the lower TP73-AS1 expression group, compared with that in the higher TP73-AS1 expression group in the 64 GC patients. (C) Overall survival rate was statistically higher in the lower TP73-AS1 expression group, compared with that in the higher TP73-AS1 expression group in the 64 GC patients. (D) TP73-AS1 expression was higher in MGC803 and BGC-823 cells than that in GES-1 cells. *P<0.05, **P<0.01. GC, gastric cancer.