Lyme IgG Immunoblot Showing Reactions of Rabbit Antisera to Different BBsl and TBRF Borrelia species. Specific rabbit antisera produced against different BBsl (lanes 1–7) and TBRF (lanes 8 and 9). Borrelia species were tested individually on Lyme IgG IBs: Lane 1—B. burgdorferi ss B31, 2—B. burgdorferi ss 297, 3—B. afzelii, 4—B. garinii, 5—B. californiensis, 6—B. spielmanii, 7—B. valensiana, 8—B. hermsii, and 9—B. coriaceae. P—positive control human serum, N—negative control human serum, 1 Control—conjugate control, and 2 Control—serum control.