PMC

Intensity profiles of DAPI, Cx43, WGA, α-SMA, and vimentin through MI to MI border zone to distal tissue. The profiles were generated from the image stacks represented in Figure .

Pie charts of volume fractions in (A) control, (B) region 1, (C) region 2, and (D) region 3. For statistical information and significance of differences see Table .

Evaluation of fibrosis. (A) Sum of fractional volumes of domains contributing to fibrosis. (B) Degree of fibrosis in proximal, adjacent and distal regions to the MI calculated based on Equation (1). Brackets mark significant differences between groups.

3D reconstruction of control myocardium with (A) cardiomyocytes, (B) complementary tissue constituents, (C) extracellular space, (D) fibroblasts, and (E) vessels. Volume fractions of tissue constituents are shown in (F). The reconstruction has a size of 204.8 × 204.8 × 41.2 μm.

3D reconstruction of tissue in region 1 with (A) cardiomyocytes, (B) complementary tissue constituents, (C) extracellular space, (D) fibroblasts, (E) myofibroblasts, and (F) vessels. Volume fractions of tissue constituents are shown in (G). The reconstruction has a size of 204.8 × 204.8 × 41.2 μm.

3D reconstruction of tissue in region 3 with (A) cardiomyocytes, (B) complementary tissue constituents, (C) extracellular space, (D) fibroblasts, (E) myofibroblasts, and (F) vessels. Volume fractions of tissue constituents are shown in (G). The reconstruction has a size of 204.8 × 204.8 × 41.2 μm.

Statistical analyses of the relationship between volume fractions of tissue constituents and calculated conductivities. Each symbol represents an image-based computation of conductivity. Linear regression modeled the relationship between V_e and (A) σ_e,l, (B) σ_e,t and (C) σ_e,n, V_e–V_cleft and (D) σ_e,l, (E) σ_e,t and (F) σ_e,n as well as between V_cleft and (G) σ_e,l, (H) σ_e,t, and (I) σ_e,n.

Image-based computation of extracellular conductivity tensor for control tissue. 3D-reconstructions of the extracellular space with modeled electrodes for applying voltages in (A) x, (B) y, and (C) z-direction. Only half of the image stacks is shown. (D–F) Calculated potential distribution corresponding to (A–C). (G–I) Calculated current density corresponding to (D–F).

Image-based computation of extracellular conductivity tensor for tissue in region 3. 3D-reconstructions of the extracellular space with modeled electrodes for applying voltages in (A) x, (B) y, and (C) z-direction. Only half of the image stacks is shown. (D–F) Calculated potential distribution corresponding to (A–C). (G–I) Calculated current density corresponding to (D–F).

Image-based computation of extracellular conductivity tensor for tissue in region 1. 3D-reconstructions of the extracellular space with modeled electrodes for applying voltages in (A) x, (B) y, and (C) z-direction. Only half of the image stacks is shown. (D–F) Calculated potential distribution corresponding to (A–C). (G–I) Calculated current density corresponding to (D–F).

Distribution of (A) WGA, (E) DAPI, (I) Cx43, (M) α-SMA, and (Q) vimentin in a single slice of an image stack from MI heart. Scar to region farthest from scar is shown from left to right. Zoom-in of regions identified in panel (A) are shown for each channel. Scale bar in (A) 50 μm. Applies to (E,I,M,Q). Scale bar in (B) 20 μm. Applies to (C,D, F–H, J–L, N–P, R–T).