PMC

Immunofluorescence staining of gpNMB in MDA-MB-468 (A) and MDA-MB-231 (B) tumor sections. Scale bars represent 50 μm. DAPI stained nuclei are marked blue and gpNMB stained (positive) membrane fragments are shown as green.

(A) Maximum Intensity Projections of WHIM-4E and WHIM-5F. Mean SUV are shown as values next to the tumors. (B) Correlative study between mean SUV in the tumor and gpNMB expression via ELISA.

(A) Maximum Intensity Projections of representative mice. (B) Mean SUV in tumors at 4 and 7 days show significantly higher [⁸⁹Zr]DFO-CR011 uptake in MDA-MB-468 at both time points. (C) Biodistribution study post PET imaging at 7 days. Similar [⁸⁹Zr]DFO-CR011 uptake in normal organs were observed for both xenograft models.

(A) Competitive binding study in MDA-MB-468. (B) Cell uptake and blocking studies in cell lines with varying gpNMB expression. (C) The cell uptake of [⁸⁹Zr]DFO-CR011 is correlated with gpNMB expression in whole cell lysates (Pearson’s r = 0.95). (D) Rate of cellular internalization at 37°C showed 50% of bound [⁸⁹Zr]DFO-CR011 internalized within 30 – 60 min. The reaction at 4°C significantly reduced cellular internalization.