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3.
Figure 2

Figure 2. The trend of WBCs and inflammatory cytokines in rabbits of the mERAT group, the appendectomy group and the blank group on days 1, 2, 3, 5, 7, and 9. From: The immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute appendicitis.

A. The number of WBCs. B. Levels of CRP were determined by Elisa. C. Levels of TNF-α. D. Levels of IL-6. E. Levels of IL-4. F. Levels of IL-10. (A-F) *P < 0.05, **P < 0.01, NS = no significance.

Suqin Liu, et al. Oncotarget. 2017 Sep 12;8(39):66528-66539.
4.
Figure 6

Figure 6. Relative expression of TLR4, MYD88, and NF-κB on the 1st, 2nd, 3rd, 5th, 7th, 9th day in rabbits of the mERAT group, the appendectomy group and the blank group. From: The immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute appendicitis.

A. Relative mRNA expression of TLR4 was detected by qPCR. B. Relative mRNA expression of MYD88. C. Relative expression of NF-κB in nuclear and cytoplasmic protein was determined by western blot in rabbits of the mERAT group. LaminB (64KDa), β-actin (42KDa), NF-κB p65 (60KDa). D. Relative expression of NF-κB in nuclear and cytoplasmic protein in rabbits of the appendectomy group. E. Relative expression of NF-κB in nuclear and cytoplasmic protein in rabbits of the blank group.

Suqin Liu, et al. Oncotarget. 2017 Sep 12;8(39):66528-66539.
5.
Figure 4

Figure 4. The composition of fecal microbiota before modeling and at 2, 4, 8 weeks after treatment in rabbits of the mERAT group, the appendectomy group and the blank group. From: The immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute appendicitis.

A.-F. Quantitative real-time PCR was used to determine fecal microbiota in rabbits of the three groups. Values are shown as a relative ratio to total bacterial 16s rRNA measured by 2-∆∆ct method. A.-F. *P < 0.05, **P < 0.01, NS = no significance.

Suqin Liu, et al. Oncotarget. 2017 Sep 12;8(39):66528-66539.
6.
Figure 5

Figure 5. The number of IgA+, IgG+, IgM+, CD4+, Ki67+, and CD8+ cells in the appendix before modeling and at 2, 4, 8 weeks after treatment in rabbits of the mERAT group and the blank group. From: The immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute appendicitis.

A. The number of IgA+ cells. B. The number of IgG+ cells. C. The number of IgM+ cells. D. The number of CD4+ cells. E. The number of Ki67+ cells. F. The number of CD8+ cells; CD8+ cells in the appendix before modeling and at 2 ,4 , 8 weeks after mERAT (from left to right, black arrow); CD8+ cells in the appendix in the blank group at the same time point as the mERAT group (from left to right, black arrow). (A-F) *P < 0.05, NS = no significance. Bar = 3μm.

Suqin Liu, et al. Oncotarget. 2017 Sep 12;8(39):66528-66539.

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