PMC

Functional classification of immunity-related DEGs in response to destruxin A.

Screening of immunity-related DEGs in response to destruxin A at 2 h, 4 h, and 6 h post-injection.

Screening of top ten immunity-related DEGs persistently expressed in response to destruxin A at 2 h, 4 h, and 6 h post-injection.

Summary of Gene ontology annotation. Functional classification of immunity- related DEGs at 2 h, 4 h, and 6 h post-infection in P. xylostella using gene ontology terms.

KEGG pathway annotation classification of immunity-related genes in P. xylostella infected with destruxin A at 2 h, 4 h, and 6 h. The abscissa is the KEGG classification, and the ordinate left is the gene number.

A Venn diagram of immunity-related differentially expressed genes in P. xylostella at 2 h, 4 h, and 6 h post-injection. The numbers in each circle show immunity-related differentially expressed genes in each comparison treatment and the overlapping regions display genes that are commonly expressed among the comparison treatments.

Expression of differentially expressed immunity-related genes at different time courses after destruxin A injection. Each vertical bar represents the mean ± SEM (n = 3) for various time points. Statistically significant differences in different groups are indicated by different letters at P < 0.05.

Scatter plot of all expressed genes in Plutella xylostella at 2 h, 4 h, and 6 h post-injection. X-axis and Y-axis present log2 value of gene expression. Blue means down-regulated genes, orange means up-regulated genes, and brown means non-regulated gene. (A) 2 h post-injection, (B) 4 h post-injection, (C) 6 h post-injection.

Validation of differential expression ratio (log2) achieved by RT-qPCR and RNA-Seq for immunity-related genes. Cecropin2, Cecropin (Px_105394859); Gloverin1, Gloverin (Px_105389810); Moricin3, Moricin (Px_105392532); Lectin2, Lectin (Px_105392416); SP1, Serine Protease (Px_105381787); KTI, Kazal-type Inhibitor (Px_105382984); STAT, STAT (Px_105396563); Tryp-SPN2, Trypsin-like Serine Proteinase (Px_105393249); MAPK1, Mitogen-activated Protein Kinase (Px_105380044); PPO1, Prophenoloxidase (Px_105393828); Transferrin1, Transferrin (Px_105384728); Peroxidase1, Peroxidase (Px_105388497); SPI3, Serine Protease Inhibitor (Px_105389206); Cactus, Cactus (Px_105384975); Serpin4, Serpin (Px_105396589).