PMC

Flow chart describing the FECSU cohort and the selection of study subjects.

Bacteria carrying clbA are abundant in stool of CRC patients. Differences in absolute number (n) and percentage (%) of (a) clbA‐ and (b) afaC‐positive stool samples between controls, and patients diagnosed with dysplasia or cancer are illustrated.

Increased levels of F. nucleatum are detected in stool of CRC patients. (a) A Beeswarm Boxplot is used to illustrate the relative levels of F. nucleatum in stool of control patients, and patients diagnosed with dysplasia or cancer. Horisontal lines indicate median (in bold) and quartiles. (b) An ROC curve displaying the specificity and the sensitivity for the F. nucleatum assay. The ROC curve was calculated using the variable for F. nucleatum and cancer/no cancer. The level of F. nucleatum in each sample is given as a relative quantification with the total microbial 16S rRNA gene DNA in each sample as reference 2^{∧ (−ΔCq)}, ΔCq = Cq^{F. nucleatum} − Cq16^{S rRNA gene}).