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5.
Fig 3

Fig 3. GO classifications of the unigene sequences.. From: Understanding the molecular mechanisms underlying the effects of light intensity on flavonoid production by RNA-seq analysis in Epimedium pseudowushanense B.L.Guo.

Annotated unique sequences were classified into ‘Biological process’, ‘Cellular component’ and ‘Molecular function’ respectively.

Junqian Pan, et al. PLoS One. 2017;12(8):e0182348.
6.
Fig 4

Fig 4. KEGG classifications of all unique sequences.. From: Understanding the molecular mechanisms underlying the effects of light intensity on flavonoid production by RNA-seq analysis in Epimedium pseudowushanense B.L.Guo.

A: Cellular Processes; B: Environmental Information Processing; C: Genetic Information Processing; D: Human Diseases; E: Metabolism and F: Organismal Systems.

Junqian Pan, et al. PLoS One. 2017;12(8):e0182348.
7.
Fig 9

Fig 9. Comparison of the expression patterns of fifteen genes obtained from RNA-seq experiments with those obtained from RT-qPCR experiments.. From: Understanding the molecular mechanisms underlying the effects of light intensity on flavonoid production by RNA-seq analysis in Epimedium pseudowushanense B.L.Guo.

Relative expression values, normalized to actin, were shown as 2−ΔΔCt relative to mean of the L, M and H treatments. Error bars represent the SD of three biological replicas with three technical replicas each.

Junqian Pan, et al. PLoS One. 2017;12(8):e0182348.
8.
Fig 1

Fig 1. The amounts of Epimedin A, Epimedin B, Epimedin C and Icariin in E. pseudowushanense under different light intensities.. From: Understanding the molecular mechanisms underlying the effects of light intensity on flavonoid production by RNA-seq analysis in Epimedium pseudowushanense B.L.Guo.

The data are expressed as the mean±SD. Different letters (a, b and c) indicate significant differences among groups with different light intensity treatments (p≤0.05; n = 30).

Junqian Pan, et al. PLoS One. 2017;12(8):e0182348.
9.
Fig 10

Fig 10. RT-qPCR analysis of differential expression genes involved in flavonoids biosynthesis in E. pseudowushanense at different light intensities.. From: Understanding the molecular mechanisms underlying the effects of light intensity on flavonoid production by RNA-seq analysis in Epimedium pseudowushanense B.L.Guo.

Relative expression values, normalized to actin, were shown as 2−ΔΔCt relative to mean of the five light treatments. Error bars represent the SD of three biological replicas with three technical replicas each.

Junqian Pan, et al. PLoS One. 2017;12(8):e0182348.
10.
Fig 7

Fig 7. Volcano plot showing the distribution of log10(p value) vs. log2(Fold Change).. From: Understanding the molecular mechanisms underlying the effects of light intensity on flavonoid production by RNA-seq analysis in Epimedium pseudowushanense B.L.Guo.

The horizontal axis represents the log2(Fold Change) between the two samples indicated on the top or on the right of the figure, while the vertical axis represents the log10(p value) for the differential expressions between the two samples. Each point represent a gene, red points indicate p value ≤ 0.05, while blue points indicate that p value > 0.05.

Junqian Pan, et al. PLoS One. 2017;12(8):e0182348.
11.
Fig 8

Fig 8. Hierarchical clustering of 21 flavonoid biosynthesis genes.. From: Understanding the molecular mechanisms underlying the effects of light intensity on flavonoid production by RNA-seq analysis in Epimedium pseudowushanense B.L.Guo.

Red, green and black colors in the heat-map represent relative gene expression levels. The scale bar denotes the log (FPKM+1)/(mean expression levels across the three treatment groups). The green color indicates down-regulated expression compared with the mean expression levels, and the red color indicates up-regulated expression compared to the mean. Three clusters (I, II and III) were indicated with brackets.

Junqian Pan, et al. PLoS One. 2017;12(8):e0182348.

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