PMC

P53, P21 and Bcl-2 genes expression in SKOV-3 cells were investigated using RT-PCR analysis (). The genes Ct values were normalized against mRNA level of β-actin as the housekeeping gene and the relative expression for each group was measured. After 48 hours of ginger treatment, the level of p53 expression was increased.

Tree induction algorithms also underlined the significance of features that weighed most in weighting models. Remarkably, decision tree models appointed the same features selected by attribute weighting as the root features to build the trees, as can be seen in . The trees were just single branches showing the selected features were so decisive that can be used as cut off criteria.

Among the studied tumor repressor genes, p53 was the top highly upregulated transcription factor in response to ginger extract, additional systems biology and meta-analysis were performed to unravel possible involved mechanism of ginger action through p53. Here, rank of correlation value was used rather than correlation value due to its reliability in meta-analysis. The top 100 co-expressed genes with p53 (Tp53) sorted based on low MR are presented in . The co-expression network, derived based on calculated association coefficients, are presented in .

In order to determine the effect of ginger on the SKOV-3 cell lines proliferation, MTT assay was illustrated at 24, 48 and 72 hours after ginger treatment. As shown in and cell growth was inhibited considerably by ginger; consequently, it can be seen in figures, cell proliferation was decreased to 50% (P<0.05) after 48 and 72 hours of treatment. The results from analysis of the data for cell viability assay via MTT demonstrated that at 24h, 48h and 72h time points, the IC50 of ginger for SKOV-3 was approximately 97 µg/ml, 60 µg/ml and 40 µg/ml. respectively.

In order to determine the effect of ginger on the SKOV-3 cell lines proliferation, MTT assay was illustrated at 24, 48 and 72 hours after ginger treatment. As shown in and cell growth was inhibited considerably by ginger; consequently, it can be seen in figures, cell proliferation was decreased to 50% (P<0.05) after 48 and 72 hours of treatment. The results from analysis of the data for cell viability assay via MTT demonstrated that at 24h, 48h and 72h time points, the IC50 of ginger for SKOV-3 was approximately 97 µg/ml, 60 µg/ml and 40 µg/ml. respectively.