PMC

Schematic of the decolorization reactor setup.

The number of isolates from Lakes Abaya and Chamo over time.

The effects of pH (a) and temperature (b) on decolorization efficiency.

The effects of different nutritional supplements [(a) carbon sources, (b) nitrogen sources] on decolorization efficiency.

Effects of (a) initial dye concentration and (b) dye types on decolorization efficiency. Reactive Red 120 (RR 120), Reactive Red 141 (RR 141), Reactive Red 239 (RR 239), Reactive Blue 19 (RB 19), Reactive Blue 198 (RB 198), Reactive Yellow 84 (RY 84) and Reactive Yellow 160 (RY 160).

Bacillus sp. strain CH12 and related organisms were aligned based on 16S rRNA gene sequences retrieved from NCBI GenBank with neighbour-joining method. The triangle filled indicates strain CH12 isolated from Chamo Lake in this study. Bootstrap values based on 1000 replications are listed as percentages at the branching points (values ≥50% shown at the node). Scale bar, 0.1 is the number of nucleotide changes per sequence position.