PMC

Length distribution of tags in the small RNA libraries. The relative abundance of small RNA with length ranging from 18–30 nt are shown in the graph.

Gene ontology (GO) of predicted targets by differentially expressed miRNAs under PVY infection. (A) Category of cellular components; (B) Category of molecular functions;(C) Category of biological process.

Gene ontology (GO) analysis of differentially expressed mRNAs under PVY infection in tobacco. (A) Category of cellular components; (B) Category of molecular functions; (C) Category of biological processes.

Combined analysis of miRNA and mRNA expression network under PVY infection.(A) Positive regulation of miRNAs versus their target genes; (B) Negative regulation of miRNAs versus their target genes.The triangle represents mRNA, circle represents miRNA, green and red represent down regulate and up regulate, respectively.

Differentially expressed mRNAs in PVY-infected and mock-inoculated tobacco plants. (A) Gene density of mRNAs in the PVY-infected (three biological replicates: A1, A2, A3) and mock-inoculated (three biological replicates: B1, B2, B3) tobacco RNA libraries; (B) Total numbers of up-regulated and down-regulated DEGs; (C) Differentially expressed mRNAs in PVY-infected (A1, A2, A3) and mock-inoculated (B1, B2, B3) tobacco. Every row shows a different gene. Red, black and green indicates expression levels of mRNA slow, medium and high, respectively.

Virus-derived siRNA (vsiRNA) from PVY targets NtTCTP mRNA and mideates plant resistance to PVY infection.(A) VsiRNA from PVY targets NtTCTP mRNA, x-axis represents the read counts of degradome sequencing for the genes that predicted as the targets of vsiRNA from PVY genome, y-axis represents vsiRNA reads from PVY genome;(B) The incidence rate of NtTCTP overexpressing lines O2, O7,wild type and NtTCTP silencing lines Ri16, Ri20; (C) Typical symptoms of NtTCTP overexpressing lines O2, O7,wild type and NtTCTP silencing lines Ri16, Ri20.(D) Virus concentration of healthy plant (H), NtTCTP overexpressing lines O2, O7,wild type and NtTCTP silencing lines Ri16, Ri20 detected by ELISA.