PMC

After analysis of the results from the exploratory cohort, 46 patients were prospectively recruited with consecutive enrollment into the confirmatory cohort that underwent additional pre-and postoperative assessment of cortisol production. APA, unilateral aldosterone-producing adenoma; ACTH, adrenocorticotropic hormone; BAH, bilateral adrenal hyperplasia; HOMA-IR, homeostasis model assessment of insulin resistance.

A tissue microarray with adenoma tissue from 57 patients was studied by immunohistochemistry for expression of CYP11B1, required for cortisol and 11β-hydroxyandrostenedione synthesis, and CYP11B2, the enzyme responsible for aldosterone synthesis, followed by digital image analysis for quantification of staining intensity. Representative immunohistochemistry examples from 2 patients with in vivo glucocorticoid excretion within the lowest and highest quartiles, respectively, are shown (total original magnification in all panels, ×20).

Steroid metabolite excretion in 24-hour urine was measured by gas chromatography-mass spectrometry in selected-ion-monitoring mode. Heatmap visualizations depict log-transformed steroid metabolite excretion values in the primary aldosteronism patients (group: black) in comparison to 162 healthy controls (group: white). (A) Mineralocorticoid and mineralocorticoid precursor metabolites ordered according to increasing amounts of tetrahydroaldosterone (THAldo) excretion. (B) Glucocorticoid metabolites in order of increasing amounts of cortisol excretion. Scale and color code were chosen separately for each panel according to the respective range of observed values. THA, tetrahydro-11-dehydrocorticosterone; THB, tetrahydrocorticosterone; THDOC, tetrahydro-11-deoxycorticosterone; THF, tetrahydrocortisol; THE, tetrahydrocortisone.

The panels show the 24-hour urinary excretion of tetrahydroaldosterone (A), cortisol (B), total glucocorticoid metabolites (C), and the major adrenal androgen metabolite 11β-hydroxyandrosterone (D) in primary aldosteronism patients (PA; n = 174) in comparison to healthy controls (Co; n = 162), patients with endocrine-inactive adrenal adenoma (EIA; n = 56), patients with subclinical Cushing’s (differentiated into 2 groups: SC1 (n = 55), morning cortisol after 1 mg dexamethasone overnight > 50 and < 138 nmol/l; SC2 (n = 49), morning cortisol >138 nmol/l), and overt adrenal Cushing’s syndrome patients (Cu; n = 47). Boxes represent median and interquartile range, whiskers represent 5th and 95th centiles. **P < 0.01 versus controls, ***P < 0.001 versus controls. Comparisons between groups were made with linear regression models to adjust for age and sex in comparisons between all 6 groups.

The panels show 24-hour urinary excretion of tetrahydroaldosterone (A), cortisol (B), total glucocorticoid metabolites (C), and 11β-hydroxyandrosterone (D). ***P < 0.001 versus controls (Co); ⁺⁺⁺P < 0.001 versus preoperative. Boxes represent median and interquartile range, whiskers represent 5th and 95th centiles. Panels E and F show individual values and mean ± SEM for serum cortisol at baseline and 30 minutes after cosyntropin stimulation in 46 primary aldosteronism patients tested 2 weeks postoperatively, in comparison to 82 healthy controls. The dotted line represents the diagnostic cut-off for adrenal insufficiency (serum cortisol 30 minutes after 250 μg cosyntropin < 15.6 μg/dl [<430 nmol/l], equivalent to the 5th centile of the cortisol response in the 82 healthy controls). ***P < 0.001 versus controls. Comparisons of steroid excretion before and after unilateral adrenalectomy were made using 2-sided Wilcoxon’s signed-rank test. Linear models were also fitted comparing preoperative and postoperative log-transformed steroid-metabolite measures to controls, adjusting for age and sex.