PMC

Discarded: N = 1 AB larva and N = 3 TL larvae as they showed no clear response to the first (and subsequent) stimulus (<15mm/s; see also ).

Panel A: Distance moved (means + SEMs) for AB and TL larvae across the different light-dark periods (for statistics see text; between strains: Student’s t-test; *: p ≤ 0.05, **: p ≤ 0.01, ***: p ≤ 0.001). Panel B: Change in maximum velocity (Δ of the first 30s of the light period and the last 30s of the dark period; means + SEMs) from dark to light for AB and TL larvae (statistics: values different from 0 (i.e. no change) per strain (one-sample t-test): *: p ≤ 0.05 (1-tailed values [,])). Panel C: Change in distance moved (Δ of the first 30s of the dark period and the last 30s of the light period; means + SEMs) from light to dark for AB and TL larvae (statistics: values different from 0 (i.e. no change) per strain (one-sample t-test): ***: p ≤ 0.001 (1-tailed values [,]); between strains: Student’s t-test; *: p ≤ 0.05).

Panel A: Acoustic/Vibrational startle responses of 5 dpf AB larvae. Mean values of maximum velocity (Vmax; mm/s) following 10 acoustic/vibrational stimuli and periods without stimuli (10 stimuli; 20s ISI) for AB larvae (N = 1 larva was discarded as it showed no clear response to the first (and subsequent) stimulus (~2 mm/s)). Panel B: Acoustic/Vibrational startle responses of 5 dpf TL larvae. Mean values of maximum velocity (Vmax; mm/s) following 10 acoustic/vibrational stimuli and periods without stimuli (10 stimuli; 20s ISI) for TL larvae (N = 2 larvae were discarded as they showed no clear response to the first (and subsequent) stimulus (~11 and ~2 mm/s)). Panel C: Mean (+SEM) values of maximum velocity (Vmax; mm/s) during 10 acoustic/vibrational stimuli with a 20s ISI (peaks of panels A and B) of AB and TL larvae (statistics: between AB and TL larvae: Student’s t-test: *: p ≤ 0.05, **: p ≤ 0.01, ***: p ≤ 0.001).