PMC

(A) IHC staining of FAT4 and p53 in different tumor regions which have been profiled by M-WES. Mutational status is indicated on top of each region. Scale bars, 100 μm. WT, wildtype. (B) Heat map of the CCF of putative driver mutations. Numeric number in each square shows the CCF. Columns, tumor regions; Rows, genes.

Phylogenetic trees were constructed from all somatic variants by Wagner parsimony method using PHYLIP (See Method). Blue, green, and orange lines represent trunk, shared branch, and private branch, respectively. Lengths of trunk and branch are proportional to their number of mutations. Putative driver events are mapped along the trees as indicated. Heat maps indicate the presence (red) or absence (grey) of a mutation in each tumor region (T) or matched nonmalignant liver tissue (N). For case HCC5647, trunk length was reduced for display purpose.

(A) Pairwise fraction analysis of truncal and branch variants on the basis of the six mutation classes. P values were derived by Paired Students’ T-test upon verification of normality and variance within each group, with those over 0.1 not shown. (B) Mutational signatures of all truncal and branch variants was inferred by deconstructSigs. Signatures are displayed according to the 96-substitution classification defined by the substitution class and sequence context (). (C) Dot plots display the contributions of individual mutational signatures to individual cases, with each dot representing one case. Signatures 1–30 were based on the Wellcome Trust Sanger Institute Mutational Signature Framework ().

(A) The proportion of clonal and subclonal mutations in both driver and passenger events. (B) CCF analysis of mutations in representative driver genes from TCGA HCC dataset. Each line represents an individual mutation. Round dot, upper and lower end of each line represents CCF, upper and lower bound of confidence interval, respectively. Clonal and subclonal CCF are shown as dark blue and orange, respectively. Driver genes were identified using MutSig algorithm () on the basis of the TCGA data (FDR q < 0.1). P values were derived by hypergeometric tests comparing the frequency of subclonal mutations in each gene against that in all driver genes. In those genes without any subclonal mutations, P value was not calculated.

(A) Unsupervised hierarchical clustering of intratumoral methylation profiling of five HCC cases. Rows of the heat maps denote the methylation levels of variably hypermethylated (upper panel) or hypomethylated (upper panel) CpG sites across different tumor regions. Columns represent samples. (B) Enrichment plots showing the distribution of both variably and invariably hypermethylated (upper panel) or hypomethylated (lower panel) CpG sites across a variety of functional genomic domains. nCGI-Prom (non CG island Promoter); CGI-nProm, (CG islands not in promoter regions); CGI-Prom, CG island promoters; nCGI-PMD, PMD excluding CGI probes. All P values of hypergeometric enrichment test (comparing the frequency of each variable and invariable probe set category to that of array background) were shown in the . (C) Dot plots displaying enriched GO biological processes for the genes associated with variably and invariably hypermethylated promoters, with each dot representing one individual case. Red line indicates P value of 0.01.