PMC

IHC images from a representative region with both tumor cell and immune cell staining. Each assay is labeled in the inset and scale bars = 200 μm.

The top chart shows the correctly predicted proportion of positive cases and cut-points color coded including >1%, >5% and >50%. The bottom chart shows the correctly predicted proportion of negative cases at each cutoff.

A) Average sores for tumor cells obtained by averaging the reads of all 13 pathologists for each of the 90 slides. Inset shows the color code for each assay. B) Frequency distributions for tumor cells each assay color coded for scoring category and summarized for percent positive at the >50% and >1% level. C) Average sores for immune cells obtained by averaging the reads of all 13 pathologists for each of the 90 slides. D) Frequency distributions immune cells for each assay color coded for scoring category and summarized for percent positive at the >10% and >1%