PMC

V, D, and J gene usage frequencies. Frequency distributions of individual VDJ gene families, and VD and DJ pairs as determined by (A) the standard method and (B) BRILIA.

Somatic hypermutations (SHM) in CDR3 and V framework regions. Comparison of the mutations accumulated in the CDR3 versus V framework (Vframe) regions, as determined by (A) the standard method and (B) BRILIA.

Comparison of somatic hypermutation (SHM) identification in real-life C57BL/6 B-cell receptor repertoires between the standard method and BRILIA. (A) Frequency distribution of SHMs per sequence predicted for all sequences in relation to their corresponding cluster’s germline sequence. (B) SHM propensity correlation returned by the standard method. Note that SHMs were determined for parent–child sequence pairs and not germline–child sequence pairs. (C) SHM propensity correlation returned by BRILIA.

BRILIA flowchart with examples and rationales for each step. For the sample sequences in the middle, the V, N_VD, D, N_DJ, and J segments are separated by a space, where a double space indicates a lack of N region (e.g., N_DJ is absent initially). A lowercase letter is either an N nucleotide (nt) or a mismatched nt with respect to the germline genes, and a bolded letter is a consensus mismatched nt.

Somatic hypermutation (SHM) hot spot analysis using BRILIA annotations. (A) Evaluation of nucleotide (nt) compositions near only mutated nts, which are at the 0 positions. The negative and positive positions are nts toward the 5′ and 3′ sides, respectively, of the 0 position nt. The nt color codes are A = red, C = green, G = blue, and T = gray. (B) Evaluation of nt compositions of all nts, regardless of whether they mutated.

Comparison of cluster counts and sizes between annotations made using the standard method and BRILIA. (A) Number of BRILIA clusters that are associated (Assoc.) with each standard cluster, where associated clusters share at least one B-cell receptor sequence. The red dots represent clusters whose corresponding lineage trees are shown in Figure . (B) Number of standard clusters that are associated with each BRILIA cluster. (C) Largest BRILIA cluster size associated with each standard cluster. The dotted diagonal line (y = x) highlights differences in the associated cluster sizes between the two methods. (D) Largest standard cluster size associated with each BRILIA cluster.

Somatic hypermutation (SHM) propensity correlations between the V and DJ segments for simulated (A) human and (B) mouse B-cell receptor sequences. The combination of color and shape of a data point represents a SHM propensity or the mutation frequency of nucleotide (nt) X₀ to nt X₁. The x- and y axes show the normalized mutation frequencies (e.g., P_A➔T + P_A➔C + P_A➔G = 1) for the V and DJ segments, respectively. R_corr is the Pearson correlation coefficient, while Slope is the slope of the linear regression line.

Somatic hypermutation (SHM) frequencies returned by BRILIA, for the purpose of evaluating SHM mechanistic models. (A) Cumulative frequency of SHM propensities for VDJ segments, excluding N regions. X₀ is the parent nt and X₁ is the child nucleotide. (B) The [A ➔ G + T ➔ C] mutation frequency (AT_mut) normalized by the total A + T content (AT_tot), plotted against the [C ➔ T + G ➔ A] mutation frequency (CG_mut) normalized by the total C + G content in the VDJ segments (CG_tot). The dotted red line, which depicts a circle with its center at the origin and a radius of 0.06, marks the mutation rate that captures 90% of the mutated sequences.

Differences in lineage trees and somatic hypermutation (SHM) frequencies between the associated standard and BRILIA clusters from the example in Figure . (A) Lineage trees are assembled from standard clusters that are subsets of a larger associated BRILIA cluster. The x-axis shows the absolute SHM distance, where the difference in SHM values between parent and child sequence is the SHM distance between the two sequences. Each dot color corresponds to a unique CDR3 sequence, and the dot size is scaled proportional to the sequence template count relative to the total template count within each lineage tree. The SHM distance is calculated based on the comparison of two 125-nucleotide sequences. Note that six single-member clusters are not drawn. (B) Lineage tree of a large BRILIA cluster that encompasses standard clusters. (C) Mutation frequencies of the V gene framework and CDR3 predicted by the two methods.