(A) Individual histopathology scores of skin changes, including inflammation, abscesses, acanthosis, ulceration and parakeratosis in wild-type mice and total score in the ears of wild-type (+/+), RORγt heterozygous (+/−) and RORγt homozygous knockouts (−/−) after saline or IL-23 intradermal injection for 7 days. (B) mRNA expression of IL-17A, IL-17F, IL-22 and TNFα in the ears of wild-type, RORγt+/− and RORγt−/−. (C-D) Individual histopathology score and representative images of skin changes in the ears of IL-23 intradermally injected wild-type mice that were orally dosed with vehicle or JNJ-54271074 at 3, 10, 30 and 60 mg/kg BID for 7 days. (E) Dose-dependent inhibition of JNJ-54271074 on mRNA expression of various cytokines in the ear. β2M was used as an endogenous control for RNA quantitation. The relative expression level was calculated based on the formula: 1.8^ (β 2M CT- Target Gene CT) * 10,000. (F) Representative flow cytometry analysis of IL-17A-producing cells in the ears and DLNs. Cells extracted from ears or DLNs of 4 to 5 vehicle- or 30 mg/kg JNJ-54271074-treated mice were pooled and stained for IL-17A+/CD3+ T cells and IL-17A+/γδ+ T cells. Values are representative of two independent experiments and are presented as the mean ± SEM, n = 4-5 mice per group. Statistical analyses were performed with one-way ANOVA (Dunnett multiple comparisons), *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.0001.