PMC

(a) PCoA of the combined cohort using BC. Arrows represent increases in the eight most abundant genera (arrow length is proportional to mean abundance; scale bar); n= 1,812. Samples are colored according to cohort. MDS1 and MDS2 are the two major axes from PCoA. (b) Correlation of age, BMI and smoking status with microbiota. For age and BMI, green arrows denote effect size (variation in β diversity explained; scale bar). Differences in smoking status are depicted as two circles with different centroids, with the dashed lines containing 50% of the samples for each group (for visualization). (c) Correlation of major nutrients with microbiota, with red arrows denoting effect size (variation in β diversity explained; scale bar). As most individual nutrients are co-linear with total energy, all arrows, save for the one for total energy, show the increase in standardized nutrient values (calculated by nutrient/total energy).

(a) Effect sizes (variation in β diversity explained) for the 42 significant loci (lead SNPs) are shown in decreasing order (left axis), and additive effects (Online Methods) are shown by the dashed line (right axis). (b) Chromosomes on the right side of the plot show the chromosomal position of genes significantly associated with β diversity (black) or an individual taxon (blue). The inner circle includes genes whose mouse homologs were implicated in one or more previously published mouse QTL studies^,–(), denoted by a link to the corresponding mouse chromosome and appearing in the same color as the human chromosome on which the gene is located. For genes located in the outer circle, either there is no mouse homolog or the mouse homolog does not fall within a QTL.

(a) LocusZoom plot of adjusted effect size (for each SNP, the actual effect size is divided by the significance threshold adjusted according to MAF category, represented by the dashed line; Online Methods) at the VDR locus, where two SNPs passed the significance threshold for association with β diversity (P < 5 × 10⁻⁸ for association with overall microbiome variation, measured by BC). (b) Association between genotypes at the lead SNP (rs7974353) and β diversity (BC). Microbiome data are shown in a PCoA plot; the dashed lines contain 50% of the samples for each group (for visualization) and show differences in the centroids for each genotype group; n = 1,812. (c) Meta-analysis in humans shows Parabacteroides to be the most significant taxon correlated with VDR using a GLM (Online Methods). The x axis shows the percentage of nonzero values for each genotype at rs7974353, and boxes and bars summarize 50% and 95% confidence intervals, respectively, for nonzero values; n = 1,812. (d) Knockout of Vdr in mice also leads to changes in Parabacteroides abundance. Error bars, 5–95% confidence intervals (n = 3 wild-type (WT) mice and n = 5 knockout mice; ). (e) LocusZoom plot for adjusted effect size in the region upstream of POMC, where 78 SNPs passed the significance threshold. (f) Association between the genotypes of the lead SNP at POMC (rs72853661) and β diversity (BC). Microbiome data are shown in a PCoA plot; the dashed lines contain 50% of the samples for each group (for visualization) and show differences in the centroids for each genotype group; n = 1,812.