PMC

(A) Simulated integration efficiency of loop 5 for the TatC homologs. (B) Loop 5 amino-acid sequence for various TatC homologs. (C) Experimental expression (black) and simulated integration efficiency (purple) for the loop 5 swap chimeras of MtTatC in which the entire loop 5 sequence of wild-type MtTatC is replaced with the corresponding sequence of other homologs. Error bars indicate the standard error of mean.

(A) Expression levels of various TatC homologs in M. smegmatis, measured by TatC-GFP fluorescence, with expression levels normalized to AaTatC (blue). (B) Simulated integration efficiency (blue, left axis) and measured expression levels in M. smegmatis (black, right axis) for a series of mutants of the Mt(Aa-tail) sequence, in which positively charged residues in the Aa-tail are mutated to alanine residues. Error bars indicate the standard error of mean.

(A) Schematic of the cytoplasmic and periplasmic topologies of the TatC C-tail with the fused β-lactamase enzyme. Misintegration of loop 7 leads to periplasmic localization of the β-lactamase, resulting in enhanced antibiotic resistance and cell survival. (B) Representative plates from the ampicillin survival test. (C) Comparison of the simulated integration efficiency (top) and relative ampicillin survival rate (bottom) for AaTatC, MtTatC, and Mt(Aa-tail). The reported cell survival corresponds to the ratio of counted cells post-treatment versus prior to treatment with ampicillin; all values are reported relative to MtTatC. Error bars indicate the standard error of mean.

(A) A topology representation of TatC with a GFP C-terminal tag, as used in the expression studies. TMDs and loops are indicated in colors and gray, respectively, and are numbered. (B) Expression levels of various TatC homologs in E. coli, measured by TatC-GFP fluorescence, with expression levels normalized to AaTatC (blue). Error bars indicate the standard error of mean. (C) Correlation of the in-gel fluorescence quantified for each band versus the experimental expression measured by flow cytometry. Both metrics are highly correlated across multiple trials (Pearson correlation coefficient R=−0.9±0.1) with in-gel fluorescence showing the same trends in expression yield as seen by flow-cytometry. Error bars indicate the standard error of mean. See also .

(A) Measured expression levels of the AaTatC and MtTatC chimera proteins, normalized to AaTatC. Shaded bars represent wild-type TatC homologs and mutants with C-tail modifications. (B) Domain definitions used in generating the swap chimeras, with TMDs highlighted. (C) A ribbons diagram of the structure of AaTatC (pdbID 4HTS). TMDs are colored according to the highlights used in part (B). (D) For each homolog, the ratio of the measured expression level for the Aa-tail chimera to that of the corresponding wild-type sequence. (E) TatC wild-type and charge mutant C-tail sequences. Positive residues are in blue and negative residues are in red. The net charge is shown to the right of each sequence. Error bars indicate the standard error of mean.

(A) Simulated integration efficiency of the Mt(Aa-tail) chimera, as a function of scaling the charges of the C-tail residues. (B) Correlation of the ratio of the measured expression for the Aa-tail swap chimeras to that of the corresponding wild-type sequence versus the charge magnitude of the wild-type C-tail (data from and ). Linear regression yields a fit of R = −0.8±0.2. (C) Correlation of the ratio of the measured expression for the Aa-tail(−) swap chimeras to that of the corresponding wild-type sequence versus the charge magnitude of the wild-type C-tail, where the Aa-tail(−) swap chimeras include a variant of the Aa-tail with net negative charge and the same overall charge magnitude. (D) Experimental expression levels in E. coli (blue, left axis) and simulated integration efficiency (black, right axis) for a series of mutants of the Mt(Aa-tail) sequence, in which positively charged residues in the Aa-tail are mutated to alanine residues. Reported values are normalized to Mt(Aa-tail). (E) Relative ampicillin survival rate in E. coli (red, left axis) and simulated integration efficiency (black, right axis) for a series of mutants of the Mt(Aa-tail) sequence, in which positively charged residues in the Aa-tail are mutated to alanine residues. Simulation results are normalized as in part (d), while ampicillin survival is normalized to the highest survival rate (i.e., with zero charge magnitude). Error bars indicate the standard error of mean.

(A) Schematic illustration of the CG simulation model that is used to model co-translational IMP membrane integration. The amino-acid sequence of the IMP is mapped onto CG beads, with each consecutive trio of amino-acid residues in the nascent protein sequence mapped to an associated CG bead; the underlying properties of the amino-acid residues determine the interactions of the CG beads, as described in the text. (B) Simulated integration efficiency of the AaTatC, MtTatC, and Mt(Aa-tail) sequences. (C) Experimental expression of the AaTatC, MtTatC, and Mt(Aa-tail) sequences. (D) The simulated integration efficiency for individual loops of both the wild-type MtTatC sequence (black bars) and the Aa-tail swap chimera (grey bars), with loop 7 highlighted. (E) Schematic of the correct and incorrect TatC topologies observed in the simulations; misintegration of loop 7 and translocation of TMD 6 leads to an incorrect final topology for MtTatC. Error bars indicate the standard error of mean. (F) For each homolog, comparison between the experimental expression levels in E. coli and M. smegmatis and the simulated integration efficiencies, reporting the ratio of the Aa-tail chimera result to that of the corresponding wild-type sequence. Ratios exceeding unity are highlighted in green, indicating enhancement due to the Aa-tail. Values in parentheses indicate the standard error of mean. See also .