PMC

Histograms representing the distribution of intratumoral and peritumoral pH_e measured by BIRDS for (A) 9L, (B) U87, and (C) RG2 tumors. The fraction of voxels with a given pH_e value is shown for intratumoral voxels (open bars) and peritumoral voxels (filled bars).

(A) T₂-weighted image shows the tumor localization due to agent extravasation (tumor = blue outline; brain = orange outline). (B) A slice from a 3D CSI data set shows varying TmDOTP⁵⁻ levels throughout the brain (same slice as in A). (C) Examples of TmDOTP⁵⁻ resonances which indicate that the pH_e (according to ) of an intratumoral voxel (pH_e = 6.64) is lower than that of a peritumoral voxel (pH_e = 7.27).

Left column shows the T₂-weighted images, which depict the localized (A) U87 and (B) 9L tumors, respectively, due to agent extravasation (tumor = blue outline; brain = orange outline). The middle column shows representative slice from 3D CSI data sets showing varying TmDOTP⁵⁻ levels throughout the brain of (A) U87 and (B) 9L tumors, respectively. The right column shows the pH_e maps (according to ) calculated from TmDOTP⁵⁻ resonances in (A) U87 and (B) 9L tumors, respectively. shows similar data for RG2 tumor.

(A) Resonance intensities of H6 in TmDOTP⁵⁻ for intratumoral (red boxes in pH_e maps in B and C) and peritumoral (black boxes in pH_e maps in B and C) voxels at various time points during co-infusion of TmDOTP⁵⁻ with probenecid. Quantitative pH_e maps (according to ) were obtained at different TmDOTP⁵⁻ concentrations indicated by star and triangles respectively at (B) 66 minutes and (C) 78 minutes after start of probenecid/TmDOTP⁵⁻ co-infusion. See for correlation of these pH_e maps.

Summary of TmDOTP⁵⁻ biodistribution (mmol/kg) in rats that were infused with (A) 0.1 mmol/kg TmDOTP⁵⁻ and (B) 1 mmol/kg TmDOTP⁵⁻ in three experimental conditions: infusion of TmDOTP⁵⁻ alone, co-infusion of 100 mg/kg probenecid and TmDOTP⁵⁻, infusion of TmDOTP⁵⁻ in renal ligated rats. Representative spectra of H6 proton in TmDOTP⁵⁻ from the kidney (top) and cortex (bottom) samples of rats that were infused with (C) 0.1 mmol/kg TmDOTP⁵⁻ and (D) 1 mmol/kg TmDOTP⁵⁻. The weight of each tissue sample is indicated below the corresponding spectrum. The concentration of TmDOTP⁵⁻ in each sample was estimated by the intensity of each spectrum after reference and plasma corrections.