PMC

Proteins identified via LC-MS/MS over a 21-day study. Total number of proteins identified for each fungus is indicated outside of diagram. Diagram generated with Venny 2.0 [Oliveros, J.C. (2007–2015) Venny. An interactive tool for comparing lists with Venn’s diagrams. http://bioinfogp.cnb.csic.es/tools/venny/index.html].

(A) Proteins unique to each fungus based on amino acid sequence (as determined by JGI protein ID). (B) Proteins unique to each fungus based on predicted function (evaluated manually). Proteins identified via LC-MS/MS over a 21-day study. Total number of unique proteins identified for each fungus is indicated in center of circles. Abbreviations as in .

Glycoside hydrolase families (both top and bottom panels). Solid bars: Portion of experimental secretome predicted to be secreted based on genome analysis. Shaded bars: Portion not predicted to be secreted. Proteins identified via LC-MS/MS over a 21-day study and classified according to the CAZy database. Abbreviations as in .

(A, B) MEROPS peptidase families. (C) CAZy auxiliary activity families. (D) Other CAZyme families. Solid bars: Portion of experimental secretome predicted to be secreted based on genome analysis. Shaded bars: Portion not predicted to be secreted. Proteins identified via LC-MS/MS over a 21-day study and classified according to the CAZy and MEROPS databases. Abbreviations as in .

Genome analysis based on OrthoMCL (see text for details). Data are solely based on genomes and do not include experimental data. Solid bars: unique proteins predicted to be secreted. Shaded bars: unique proteins not predicted to be secreted. Colors as in Figs and . Total number of predicted proteins in each genome (including unique and non-unique): 13,469 (A. alternata); 14,745 (P. sporulosum); 14,995 (Pyrenochaeta sp.); 14,430 (Stagonospora sp.).

(A) Experimentally observed secretome. Proteins identified via LC-MS/MS over a 21-day study. (B) Portion of experimental secretome predicted to be secreted based on genome analysis (see text for further explanation). (C) Full predicted secretome based on genomes only. Total number of proteins identified for each fungus is indicated in center of circles. Abbreviations from CAZy database: AA = auxiliary activities; CBM = carbohydrate-binding module; CE = carbohydrate esterase; GH = glycoside hydrolase; GT = glucosyltransferase; PL = polysaccharide lyase.