PMC

(A) Left, conformation of a single chromosome with chromatin fiber shown in semitransparent gray and condensins shown in red; two loops are highlighted with blue. Right: the diagram showing that loop repulsion straightens chromosomal cores and extends individual loops radially. (B) Left, cross section of two parallel loop brushes placed at a short distance from each other. Right, the diagram shows that steric interactions between loops of sister chromatids lead to chromatid repulsion and segregation.
DOI: http://dx.doi.org/10.7554/eLife.14864.015

Top row, the update rules used in simulations: (A) a condensin extrudes a loop by moving the two ends along the chromosome in the opposite directions, (B) collision of condensins bound to chromosomes blocks loop extrusion on the collided sides, (C) a condensin spontaneously dissociates and the loop disassembles; (D) a condensin associates at randomly chosen site and starts extruding a loop. Bottom row, (E) we use polymer simulations to study how combined action of many loop extruding condensins changes the conformation of a long chromosome.
DOI: http://dx.doi.org/10.7554/eLife.14864.002

(A) The median separation between condensin-rich axes of two sister chromosomes (solid lines) and the mean chromosome diameter (red) as a function of time, measured in condensin residence times, as above. Depletion of topo II was simulated by a simultaneous increase of the energy barrier of fiber overlap (shown by the line color) and a 3x increase in of the radius of repulsion. (B) Entanglement of sister chromatids in simulations of topo II depletion, measured by the linking number of the condensin cores of sister chromatids.
DOI: http://dx.doi.org/10.7554/eLife.14864.008

(A) The median separation between condensin-rich axes of two sister chromosomes (blue) and the mean chromosome diameter (red) as a function of time, measured in condensin residence times, as above. The blue band shows the range between 25th and 75th percentile across 10 simulation replicas at each time point; the light blue shows 10%--90% range. (B) Entanglement of sister chromatids measured by the linking number of the condensin cores of sister chromatids; the blue bands show variability between simulation replicas, same as above. (C) Conformations of two sister chromatids in the polymer simulations (shown in red and blue) illustrate the observed segregation and disentanglement.
DOI: http://dx.doi.org/10.7554/eLife.14864.007

(A) Simulations with altered condensin abundance: '0.2x condensin', '0.5x condensin', '2x condensins' and '4x condensins' - simulations with 200, 500, 2000 and 4000 condensins, correspondingly. (B) Simulations with altered parameters of the chromatin fiber and condensins: 'Stiffer fiber' – simulations with 2x bending energy of the fiber, 'softer fiber' – simulations with 0.5x bending energy; 'wide condensins' – simulations with increased spacing between condensins at loop bases; '10 nm fiber' - simulations with reduced linear DNA of the fiber; '30 nm fiber' – simulations with increased linear DNA density of the fiber and increased fiber thickness. The vertical gray lines show the experimental observations on the linear density of DNA along the human prophase chromosome I (6.25 kb/nm) and chromosome XXII (5.0 kb/nm) (). The horizontal gray lines show the experimental data on the diameter of prophase chromosomes: 400 nm (), 600 nm () and 679 nm ().
DOI: http://dx.doi.org/10.7554/eLife.14864.005

(A) Dynamics of loop array formation. Left, the fraction of the chromosome extruded into loops (loop coverage, gray) and the mean loop size (black). The time is measured in the units of condensin residence time. Data for each curve was obtained using 10 simulation replicas; the thickness of each line shows the difference between the 10th and 90th percentile at each time point. Right, a phase diagram showing loop coverage vs mean loop length. The curve is averaged over the simulation replicas and colored by log-time. Two stages of dynamics are separated by the two vertical dashed lines corresponding to 95% coverage and 95% of the steady state mean loop length. The distinction between the two stages of compaction is especially easy to see on the phase curve. (B) Dynamics of chromosome morphology. Left: chromosome length (blue) and the mean chromosome diameter (red) shown as a function of time; the thickness of each line shows the difference between the 10th and 90th percentile at each time point. Right, a phase diagram showing chromosome length vs diameter; the curve is averaged over the simulation replicas and colored by log-time. Note that most of chromosome linear compaction is achieved during the first phase, while the second is characterized by widening of the chromosome and further three-fold shortening. (C) Snapshots of polymer simulations. Top row: the conformations of chromosomes at the beginning of simulations (left), slightly before the transition between the loop formation and maturation stages (middle), and in the steady state (right). Color (rainbow: from red to blue) shows positions of individual monomers along the chain and demonstrates that loci in compacted chromosomes are arranged linearly according to their genomic location. Bottom row: same as above, with chromosomes shown in semitransparent gray and condensins as red spheres.
DOI: http://dx.doi.org/10.7554/eLife.14864.004