A) The number of editing sites detected in RNA isolated from specific subsets of neurons expressing the Hrp48-TRIBE protein (bars labelled TRIBE) is significantly greater than the background number of endogenous editing sites (bars labelled control). Cell types examined were the core circadian pdf neuropeptide expressing cells (pdf-Gal4), dopaminergic neurons (TH-Gal4) and generic neurons (elav-Gal4). B) Venn diagram of the genes that are identified as Hrp48 targets by TRIBE in different cell types. Example genes from three categories of targets are shown 3 in (C,D,E). C) Many commonly expressed genes are identified as targets in each of the three cell types. D, E) Some genes are identified as targets in only one (or a combination of two) cell types. D) Genes that are expressed only in certain cell types are identified as targets and (E) commonly expressed genes may be identified as a target in one cell type and not the others (dashed insets in B). Note that ‘expression’ here is classified as sufficient sequencing depth at the editing site location, and as such the numbers of cell-specifically expressed genes are likely an overestimation. (C,D,E) Tracks shown are; RNA-seq and Hrp48 CLIP from whole fly heads, RNA-seq and Hrp48-TRIBE editing tracks from indicated isolated neuron subtype, either GFP expressing control (labelled pdf, TH, elav) or Hrp48-TRIBE expressing cells (labelled pdf, TH, elav-TRIBE). Editing events are indicated by black bars, and the height of the bar indicates the percentage editing at that site. The scale for mRNA-seq is constant for each gene, resulting in truncation of signal of pdf in pdf cells.
See also , and .