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1.
Figure 4

Figure 4. Measured defocus variation within and across tiltseries. From: Alignment Algorithms and Per-Particle CTF Correction for Single Particle Cryo-Electron Tomography.

Plots showing large measured defocus variations within and across six VEEV cryoET tiltseries collected using a JEM3200FSC electron microscope.

Jesús G. Galaz-Montoya, et al. J Struct Biol. ;194(3):383-394.

CitationFull text

2.
Figure 6

Figure 6. CTF-Corrected Subtomogram Average of VEEV. From: Alignment Algorithms and Per-Particle CTF Correction for Single Particle Cryo-Electron Tomography.

Isosurfaces of VEEV subtomogram average comprised of the top 75% best-correlating subtomograms showing the outer glycoprotein shell (top) and the nucleocapsid (bottom).

Jesús G. Galaz-Montoya, et al. J Struct Biol. ;194(3):383-394.

CitationFull text

3.
Figure 1

Figure 1. Drift correction for cryoET images of a tilted specimen collected with a DE20 camera. From: Alignment Algorithms and Per-Particle CTF Correction for Single Particle Cryo-Electron Tomography.

Examples of VEEV images exhibiting significant drift, taken at different tilt angles, shown before (top) and after (bottom) drift correction.

Jesús G. Galaz-Montoya, et al. J Struct Biol. ;194(3):383-394.

CitationFull text

4.
Figure 2

Figure 2. Quantification of image drift in cryoET images of a tilted specimen. From: Alignment Algorithms and Per-Particle CTF Correction for Single Particle Cryo-Electron Tomography.

Examples of total drift per frame and average drift per image for all images in (A) a “bad” unidirectional tiltseries and (B) a “good” bidirectional tiltseries.

Jesús G. Galaz-Montoya, et al. J Struct Biol. ;194(3):383-394.

CitationFull text

5.
Figure 5

Figure 5. Resolution analyses of CTF-Corrected VEEV subtomogram averages. From: Alignment Algorithms and Per-Particle CTF Correction for Single Particle Cryo-Electron Tomography.

(A) Per-tomogram FSC curves for six CTF-corrected VEEV subtomogram averages from independent tomograms keeping the top 75% best correlating particles in each average. (B) FSCs for CTF-corrected subtomogram averages comprised of different fractions of particles (~100% / 516 particles; ~90% / 464 particles; ~75% / 387 particles; ~30% / 154 particles; ~10% / 51 particles).

Jesús G. Galaz-Montoya, et al. J Struct Biol. ;194(3):383-394.

CitationFull text

6.
Figure 3

Figure 3. Interactive CTF fitting in EMAN2. From: Alignment Algorithms and Per-Particle CTF Correction for Single Particle Cryo-Electron Tomography.

Examples of periodogram averaging by interactive micrograph tiling for power spectrum (PS) computation and CTF fitting directly from the imaging area for cryoET images of a tilted specimen. (A) Tiling of 0° image from a VEEV tiltseries showing clear CTF ripples out to 4/5 Nyquist. (B) Example of per-strip tiling of a high tilt (50°) image from a VEEV tiltseries, showing clear CTF ripples out to 2/3 Nyquist.

Jesús G. Galaz-Montoya, et al. J Struct Biol. ;194(3):383-394.

CitationFull text

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