a, Comparable learning curves between WT and KO mice during cued training protocol. b, Intact spatial learning demonstrated in 24hr probe trial. c, Ptchd1 KO mice show normal reversal learning curve. d, No significant difference between WT and KO mice in 24 hour probe trial after reversal learning protocol (10 WT, 10 KO). e, Representative images of WT (black) and KO (red) strides. Forepaw position is represented by green paint and hindpaw position is represented by pink paint (scale bar = 2cm). Quantification reveals elongated stride length and width (10 WT, 11 KO). f, KO mice show drastic reductions in grip strength as measured by the hanging wire test (12 WT, 11 KO). g–h, KO mice attack intruder mice for a longer duration (g) and with a shorter latency to attack (h) in the resident-intruder test for aggression (10 WT, 10 KO). Two-way repeated measures ANOVA with Bonferroni post-hoc tests (a, c), one-way ANOVA with Bonferroni multiple comparison tests (b, d), two-tailed t-tests (e, f) and Wilcoxon ranksum tests (g, h) were used for statistical analysis. Mean ± s.e.m. (a, e), mean (b, d, e–f), median (g–h). *P<0.05; **P<0.01; ***P<0.001; N.S. = not significant.