PMC

Histological and immunohistochemical staining of representative cases of chronic gastritis with lymphoid aggregates and MALT lymphoma with lymphoepithelial lesions (LEL) or lymphoepithelial destruction (LED); H&E staining, B220 labeling B-cells and anti-cytokeratine staining labeling the epithelium. Centrocyte-like cells infiltrate the gastric epithelium (arrows). Scale bars show 20 μm. Original magnification x40.

Sera from infected mice were collected 12 weeks after infection with H. felis and antibody titers were determined by ELISA. (A-B) Total immunoglobulin levels were elevated to similar extent in both infected genotypes as compared to their uninfected littermates while (C, D) H. felis-specific immunoglobulin levels were decreased in infected Plcg2^Ali5/+ mice. Data represent mean ± SEM of 3 independent experiments including n = 14 to 15 infected Plcg2^Ali5/+ mice and n = 15 infected WT mice. Two independent experiments were done for uninfected mice (n = 4 per genotype). Data were analyzed by using the Mann-Whitney U-test. LU = labor units; ns = not significant. *p ≤ 0.05; **p ≤ 0.01.

Histological scoring of Foxp3⁺ Tregs in spleen of (A) uninfected and (B) infected mice. Plcg2^Ali5/+ and WT mice (n = 5 per genotype for uninfected mice; n = 10 infected Plcg2^Ali5/+ mice, n = 11 infected WT mice) were used to count Foxp3⁺ Tregs in the T-cell area of the white pulp. Foxp3⁺ Tregs were determined on a CD3⁺ T-cell area of 1000 μm². (C-D) Relative proportion of Tregs and ecto-5`-nucleotidase (CD73) expressing Tregs of CD4⁺ spleen cells from uninfected Plcg2^Ali5/+ and WT mice. Mouse spleen MNCs were stained with Mouse Regulatory T-cell Staining Kit #2 and co-stained with anti-CD73. Cells were analyzed by flow cytometry for CD4⁺/CD25⁺/Foxp3⁺ Tregs and CD73⁺ expressing Tregs determined from CD4⁺ T-cells. A representative of two to three independent experiments with three to four mice per genotype is shown. Distribution of (C) CD4⁺/CD25⁺/Foxp3⁺ Tregs and (D) CD73⁺ expressing Tregs in Plcg2^Ali5/+ and WT mice are shown in percentages. Data represent mean ± SEM of 2 to 3 independent experiments and were calculated using Student´s t-test. MNCs = mononuclear cells; Tregs = regulatory T-cells. *p ≤ 0.05; **p ≤ 0.01.

(A-B) Proliferation of primary spleen B-cells was measured using the BrdU flow Kit. Purified CD45R/B220⁺ B-cells were stimulated for 48 h with LPS, CpG, α-IgM or α-CD40 + IL-4 or left unstimulated. Cell proliferation was assessed by flow cytometry after pulse with BrdU for the last 18 h of culture. Stimulation of primary spleen B-cells of (A) H. felis-infected mice (infection time for 12 weeks) or (B) uninfected mice showed no differences in cell proliferation. Data show live cells as mean ± SEM including n = 6 infected mice/genotype and n = 3 uninfected mice/genotype. (C-D) IgG1 class switching through stimulation of B-cells of non-infected mice in vitro. Purified CD43^- spleen B-cells and total mLN cells were stimulated with LPS, with or without IL-4. 4 days after stimulation cells were stained with anti-IgG1 and B-cells were counterstained with anti-CD45R/B220. Cells were analyzed by flow cytometry and membrane IgG1 positive B-cells of (C) spleen and (D) mLN B-cells are shown as percentages. B-cells treated with LPS only were used as controls. Data represent mean ± SEM of 2 independent experiments including n = 6 individually examined mice/genotype. All statistical analyses were done by unpaired Student´s t-test. mLN = mesenteric lymph nodes.